{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T20:31:16Z","timestamp":1792009876782,"version":"4.4.0"},"reference-count":27,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2024,7,4]],"date-time":"2024-07-04T00:00:00Z","timestamp":1720051200000},"content-version":"vor","delay-in-days":3,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Influenza Resp Viruses"],"published-print":{"date-parts":[[2024,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      Since the outbreak of COVID\u201019, China has undertaken a variety of preventative and control measures, effectively reducing the incidence of numerous infectious diseases among the pediatric population in Hangzhou. We aim to investigate the genetic and epidemiological characteristics of\n                      <jats:italic>Human parainfluenza virus<\/jats:italic>\n                      \u20103 (HPIV\u20103) in pediatric patients during this period.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>A total of 1442 pharyngeal swab samples were collected from outpatients and inpatients with a diagnosis of acute respiratory tract infections (ARTIs) from November 2020 to March 2021. HPIV\u20103 was detected by quantitative real time polymerase chain reaction (qRT\u2010PCR). The L gene of HPIV\u20103 positive samples was amplified and sequenced.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Among 1442 children with ARTI, the positive rate of HPIV\u20103 was 7.07% (102\/1442). The positive detection rate was the highest in the 6\u2010month to 1\u2010year age group. Coinfection was observed in 36 HPIV\u20103\u2010positive samples (35.29%, 36\/102), and\n                      <jats:italic>adenovirus<\/jats:italic>\n                      (ADV) was the most common coinfecting virus (63.89%, 23\/36). The L gene of 48 HPIV\u20103 positive samples was sequenced. The nucleotide sequence analysis showed high consistency (92.10%\u201399.40%), and all strains belonged to C3a.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>During study periods, the positive detection rate of HPIV\u20103 among children is high, and the highest proportion of coinfection was observed in HPIV\u20103 mixed ADV infection. Phylogenetic analysis revealed that the nucleotide sequence of the L gene of HPIV\u20103 was highly consistent, and the main epidemic strain in this area was the C3a subtype.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/irv.13351","type":"journal-article","created":{"date-parts":[[2024,7,5]],"date-time":"2024-07-05T02:40:12Z","timestamp":1720147212000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Molecular Epidemiology of\n                    <i>Human Parainfluenza Virus Type 3<\/i>\n                    in Children With Acute Respiratory Tract Infection in Hangzhou"],"prefix":"10.1111","volume":"18","author":[{"given":"Ya\u2010jun","family":"Guo","sequence":"first","affiliation":[{"name":"Department of Clinical Laboratory, The Children's Hospital Zhejiang University School of Medicine  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lin","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases, Fujian Children's Hospital (Fujian Branch of Shanghai Children's Medical Center), National Regional Medical Center, College of Clinical Medicine for Obstetrics &amp; Gynecology and Pediatrics Fujian Medical University  Fuzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qin\u2010rui","family":"Lai","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory, The Children's Hospital Zhejiang University School of Medicine  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying\u2010shuo","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Respiratory, The Children's Hospital Zhejiang University School of Medicine  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7501-5707","authenticated-orcid":false,"given":"Wei","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory, The Children's Hospital Zhejiang University School of Medicine  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,7,4]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.16.2.242\u2010264.2003"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1055\/s\u20100036\u20101584798"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2021.679246"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1086\/507638"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.24580"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.coviro.2012.02.001"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12985\u2010023\u201002214\u20109"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010018\u201024767\u20104"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.000459"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2008.10.019"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1001\/jama.2020.6130"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.21037\/tp\u201021\u2010150"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12887\u2010021\u201002654\u20108"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.12968\/bjon.2017.26.11.602"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhin.2022.03.017"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcv.2020.104261"},{"issue":"12","key":"e_1_2_11_18_1","first-page":"971","article-title":"Viral Pathogens of Acute Lower Respiratory Tract Infection in China","volume":"45","author":"Tang L. F.","year":"2008","journal-title":"Indian Pediatrics"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.25660"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-0042-1756712"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471\u20102334\u201013\u201028"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.1097\/00006454\u2010200107000\u201000003"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcv.2018.08.004"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.diagmicrobio.2019.01.008"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.1086\/516137"},{"key":"e_1_2_11_26_1","doi-asserted-by":"publisher","DOI":"10.1159\/000369208"},{"key":"e_1_2_11_27_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0043893"},{"key":"e_1_2_11_28_1","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.74.13.5886\u20105895.2000"}],"container-title":["Influenza and Other Respiratory Viruses"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/irv.13351","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,7,26]],"date-time":"2024-07-26T10:13:13Z","timestamp":1721988793000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/irv.13351"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,7]]},"references-count":27,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2024,7]]}},"alternative-id":["10.1111\/irv.13351"],"URL":"https:\/\/doi.org\/10.1111\/irv.13351","archive":["Portico"],"relation":{"has-review":[{"id-type":"doi","id":"10.1111\/IRV.13351\/v2\/response1","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/IRV.13351\/v1\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/IRV.13351\/v1\/review2","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/IRV.13351\/v1\/review1","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/IRV.13351\/v2\/decision1","asserted-by":"object"}]},"ISSN":["1750-2640","1750-2659"],"issn-type":[{"value":"1750-2640","type":"print"},{"value":"1750-2659","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,7]]},"assertion":[{"value":"2024-03-15","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-06-12","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-07-04","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e13351"}}