{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T03:06:59Z","timestamp":1790996819753,"version":"4.1.0"},"reference-count":65,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2025,3,24]],"date-time":"2025-03-24T00:00:00Z","timestamp":1742774400000},"content-version":"vor","delay-in-days":23,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100010679","name":"Pasteur Institute of Iran","doi-asserted-by":"publisher","award":["Bd\u20109791"],"award-info":[{"award-number":["Bd\u20109791"]}],"id":[{"id":"10.13039\/501100010679","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Influenza Resp Viruses"],"published-print":{"date-parts":[[2025,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Four significant influenza outbreaks have occurred over the past 100\u2009years, and the 1918 influenza pandemic is the most severe. Since influenza viruses undergo antigenic evolution, they are the pathogens most likely to trigger a new pandemic shortly. Intranasal vaccination offers a promising strategy for preventing diseases triggered by respiratory viruses by eliciting an immunoglobulin A (IgA) response, limiting virus replication and transmission from the respiratory tract more efficiently than intramuscular vaccines. Combining intranasal administration and mRNA\u2010lipid nanoparticles can be an ideal strategy for limiting the extent of the next flu pandemic. This study explored the immunogenicity of intranasally delivered mRNA encapsulated in mannose\u2010histidine\u2010conjugated chitosan lipid nanoparticles (MHCS\u2010LNPs) as a vaccine against influenza A (H1N1) in BALB\/c mice. Intranasal administration of mRNA\u2010MHCS\u2010LNPs resulted in the generation of influenza A (H1N1) hemagglutinin\u2010specific neutralizing antibodies in vaccinated animals. The enzyme\u2010linked immunosorbent assay (ELISA) results indicated a notable increase in the quantity of immunoglobulin G (IgG) and IgA antibodies in serum and the bronchoalveolar lavage fluid (BALF), respectively, and exhibited influenza A\u2010specific IFN\u2010\u03b3 secretion in vaccinated mice, as well as a noticeable alteration in IL\u20105 production. Overall, this study demonstrated an effective immunogenic response against respiratory viral infections through intranasal delivery of an mRNA\u2010MHCS\u2010LNP vaccine.<\/jats:p>","DOI":"10.1111\/irv.70093","type":"journal-article","created":{"date-parts":[[2025,3,26]],"date-time":"2025-03-26T05:02:35Z","timestamp":1742965355000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Targeting Respiratory Viruses: The Efficacy of Intranasal mRNA Vaccination in Generating Protective Mucosal and Systemic Immunity Against Influenza A (H1N1)"],"prefix":"10.1111","volume":"19","author":[{"given":"Sara","family":"Yahyaei","sequence":"first","affiliation":[{"name":"Hepatitis and AIDS Department Pasteur Institute of Iran  Tehran Iran"},{"name":"Student Research Committee Pasteur Institute of Iran  Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Asghar","family":"Abdoli","sequence":"additional","affiliation":[{"name":"Hepatitis and AIDS Department Pasteur Institute of Iran  Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abbas","family":"Jamali","sequence":"additional","affiliation":[{"name":"Department of Influenza and Other Respiratory Viruses Pasteur Institute of Iran  Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ali","family":"Teimoori","sequence":"additional","affiliation":[{"name":"Department of Virology, Faculty of Medicine Hamadan University of Medical Sciences  Hamadan Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ehsan","family":"Arefian","sequence":"additional","affiliation":[{"name":"Department of Microbiology, School of Biology, College of Science University of Tehran  Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zohre","family":"Eftekhari","sequence":"additional","affiliation":[{"name":"Biotechnology Department Pasteur Institute of Iran  Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Parisa","family":"Jamur","sequence":"additional","affiliation":[{"name":"Hepatitis and AIDS Department Pasteur Institute of Iran  Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,3,24]]},"reference":[{"issue":"6","key":"e_1_2_10_2_1","doi-asserted-by":"crossref","first-page":"383","DOI":"10.1038\/s41577-019-0143-6","article-title":"The Human Antibody Response to Influenza A Virus Infection and Vaccination","volume":"19","author":"Krammer F.","year":"2019","journal-title":"Nature Reviews. 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M.","year":"2022","journal-title":"Vaccines (Basel)."},{"issue":"7830","key":"e_1_2_10_7_1","doi-asserted-by":"crossref","first-page":"567","DOI":"10.1038\/s41586-020-2622-0","article-title":"SARS\u2010CoV\u20102 mRNA Vaccine Design Enabled by Prototype Pathogen Preparedness","volume":"586","author":"Corbett K. S.","year":"2020","journal-title":"Nature"},{"issue":"24","key":"e_1_2_10_8_1","doi-asserted-by":"crossref","first-page":"2241","DOI":"10.1056\/NEJMoa2109522","article-title":"Evaluation of mRNA\u20101273 SARS\u2010CoV\u20102 Vaccine in Adolescents","volume":"385","author":"Ali K.","year":"2021","journal-title":"New England Journal of Medicine"},{"key":"e_1_2_10_9_1","unstructured":"A. B.Vogel I.Kanevsky Y.Che et\u00a0al. A Prefusion SARS\u2010CoV\u20102 Spike RNA Vaccine is Highly Immunogenic and Prevents Lung Infection in Non\u2010Human Primates. bioRxiv. 2020:2020.09.08.280818."},{"issue":"7868","key":"e_1_2_10_10_1","doi-asserted-by":"crossref","first-page":"572","DOI":"10.1038\/s41586-021-03653-6","article-title":"BNT162b2 Vaccine Induces Neutralizing Antibodies and Poly\u2010Specific T Cells in Humans","volume":"595","author":"Sahin U.","year":"2021","journal-title":"Nature"},{"issue":"3","key":"e_1_2_10_11_1","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1056\/NEJMoa2107456","article-title":"Safety, Immunogenicity, and Efficacy of the BNT162b2 Covid\u201019 Vaccine in Adolescents","volume":"385","author":"Frenck R. 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