{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T02:50:25Z","timestamp":1789613425854,"version":"build-2803163510"},"reference-count":15,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2025,9,18]],"date-time":"2025-09-18T00:00:00Z","timestamp":1758153600000},"content-version":"vor","delay-in-days":17,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100009139","name":"Deutsches Zentrum f\u00fcr Infektionsforschung","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100009139","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Influenza Resp Viruses"],"published-print":{"date-parts":[[2025,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>The emergence of resistance\u2010associated substitutions in RSV against novel monoclonal antibodies is a concern given widespread prophylactic use.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Aim<\/jats:title>\n                    <jats:p>To assess the prevalence of resistance\u2010associated substitutions in the RSV F protein against nirsevimab, clesrovimab, and palivizumab in German infants before widespread implementation of nirsevimab.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials &amp; Methods<\/jats:title>\n                    <jats:p>\n                      We sequenced the F protein of\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u20091042 RSV samples from German infants from seasons 2021\/2022 and 2022\/2023 and screened for variants in binding sites for nirsevimab (Site \u00d8), clesrovimab (Site IV), and palivizumab (Site II).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Prevalence of resistance\u2010associated substitutions was low (&lt; 1%) for all three monoclonal antibodies.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Although the current risk of infections with escape\u2010mutants appears to be low, our results underline the need for continued surveillance, as resistance\u2010conferring mutations to new mAbs circulated and may be selected under selection pressure.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/irv.70164","type":"journal-article","created":{"date-parts":[[2025,9,19]],"date-time":"2025-09-19T00:04:33Z","timestamp":1758240273000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Prevalence of Resistance\u2010Associated Mutations in RSV F Protein Against Monoclonal Antibodies Prior to Widespread Implementation: Findings From a Prospective German Pediatric Cohort"],"prefix":"10.1111","volume":"19","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1462-9569","authenticated-orcid":false,"given":"Martin","family":"Wetzke","sequence":"first","affiliation":[{"name":"Department of Pediatric Pneumology, Allergy, and Neonatology Hannover Medical School  Hannover Germany"},{"name":"German Center for Lung Research Biomedical Research in End\u2010stage and Obstructive Lung Disease Hannover (BREATH)  Hannover Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Duyen\u00a0Bao","family":"Le","sequence":"additional","affiliation":[{"name":"Institute of Virology, Medical Faculty and University Hospital D\u00fcsseldorf Heinrich Heine University D\u00fcsseldorf  D\u00fcsseldorf Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Inga","family":"Tometten","sequence":"additional","affiliation":[{"name":"Institute of Virology, Medical Faculty and University Hospital D\u00fcsseldorf Heinrich Heine University D\u00fcsseldorf  D\u00fcsseldorf Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-8041-5044","authenticated-orcid":false,"given":"Simon","family":"Ritter","sequence":"additional","affiliation":[{"name":"Department of Pediatric Pneumology, Allergy, and Neonatology Hannover Medical School  Hannover Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nadine","family":"L\u00fcbke","sequence":"additional","affiliation":[{"name":"Institute of Virology, Medical Faculty and University Hospital D\u00fcsseldorf Heinrich Heine University D\u00fcsseldorf  D\u00fcsseldorf Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J\u00f6rg","family":"Timm","sequence":"additional","affiliation":[{"name":"Institute of Virology, Medical Faculty and University Hospital D\u00fcsseldorf Heinrich Heine University D\u00fcsseldorf  D\u00fcsseldorf Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexander","family":"Dilthey","sequence":"additional","affiliation":[{"name":"Center for Digital Medicine Heinrich Heine University D\u00fcsseldorf  D\u00fcsseldorf Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcus","family":"Panning","sequence":"additional","affiliation":[{"name":"Institute of Virology, Medical Center, Faculty of Medicine University of Freiburg  Freiburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andreas","family":"Walker","sequence":"additional","affiliation":[{"name":"Institute of Virology, Medical Faculty and University Hospital D\u00fcsseldorf Heinrich Heine University D\u00fcsseldorf  D\u00fcsseldorf Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christine","family":"Happle","sequence":"additional","affiliation":[{"name":"Department of Pediatric Pneumology, Allergy, and Neonatology Hannover Medical School  Hannover Germany"},{"name":"German Center for Lung Research Biomedical Research in End\u2010stage and Obstructive Lung Disease Hannover (BREATH)  Hannover Germany"},{"name":"Excellence Cluster on Infection Research \u201cRESolving Infection Susceptibility\u201d RESIST  Hannover Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,9,18]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(05)71877-8"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa2110275"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jiae581"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(24)00455-9"},{"key":"e_1_2_11_6_1","unstructured":"FDA Highlights of Prescribing Information\u2014ENFLONSIA (Clesrovimab\u2010cfor)(2025).https:\/\/wwwgooglecom\/url?sa=t&source=web&rct=j&opi=89978449&url=https:\/\/wwwaccessdatafdagov\/drugsatfda_docs\/label\/2025\/761432s000lblpdf&ved=2ahUKEwi386Sf1IqPAxX\u20101gIHHZujBSsQFnoECCEQAQ&usg=AOvVaw0Z1UrzQPBXdXtKc32wf1dC."},{"key":"e_1_2_11_7_1","unstructured":"RKI STIKO: Prophylaxe von RSV\u2010Erkrankungen mit Nirsevimab bei Neugeborenen und S\u00e4uglingen. Epidemiologisches Bulletin 26\/2024 (2024)."},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(24)00570-X"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s15010\u2010025\u201002484\u20101"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.2807\/1560-7917.ES.2020.25.22.2000746"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2025.02.030"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-023-40057-8"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/rmv.2284"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcv.2022.105223"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jiq100"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-019-12137-1"}],"container-title":["Influenza and Other Respiratory Viruses"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/irv.70164","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,9,26]],"date-time":"2025-09-26T06:02:56Z","timestamp":1758866576000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/irv.70164"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,9]]},"references-count":15,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2025,9]]}},"alternative-id":["10.1111\/irv.70164"],"URL":"https:\/\/doi.org\/10.1111\/irv.70164","archive":["Portico"],"relation":{"has-review":[{"id-type":"doi","id":"10.1111\/IRV.70164\/v2\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/IRV.70164\/v1\/review1","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/IRV.70164\/v1\/review2","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/IRV.70164\/v2\/response1","asserted-by":"object"},{"id-type":"doi","id":"10.1111\/IRV.70164\/v1\/decision1","asserted-by":"object"}]},"ISSN":["1750-2640","1750-2659"],"issn-type":[{"value":"1750-2640","type":"print"},{"value":"1750-2659","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,9]]},"assertion":[{"value":"2025-06-17","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-08-29","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-09-18","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70164"}}