{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,12]],"date-time":"2026-08-12T14:36:13Z","timestamp":1786545373008,"version":"build-2736575974"},"reference-count":13,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2026,7,24]],"date-time":"2026-07-24T00:00:00Z","timestamp":1784851200000},"content-version":"vor","delay-in-days":23,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T00:00:00Z","timestamp":1782864000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2025YFC2311700"],"award-info":[{"award-number":["2025YFC2311700"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Influenza Resp Viruses"],"published-print":{"date-parts":[[2026,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>A 2024 human HPAI H5N1 case in British Columbia showed mixed viral populations containing HA\u2010190D (28%) and HA\u2010226H (35%) variants in tracheal aspirate sequencing. In this study, solid\u2010phase binding assay and molecular docking were used to evaluate the contribution of HA\u2010E190D\/Q226H mutations to the viral receptor profiles. Our results showed that HA\u2010E190D marginally reduced sialic acid \u03b12,3 receptors' affinity, while HA\u2010Q226H impaired both sialic acid \u03b12,3 and \u03b12,6 receptors' binding. These results demonstrate that neither mutation strengthens viral binding to human\u2010type receptors, indicating that such substitutions are unlikely to heighten the public health threat posed by the virus for now.<\/jats:p>","DOI":"10.1111\/irv.70298","type":"journal-article","created":{"date-parts":[[2026,7,25]],"date-time":"2026-07-25T01:29:18Z","timestamp":1784942958000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Contribution of E190D and Q226H Mutations in HA to the Receptor\u2010Binding Profile of D1.1 Genotype H5N1 Viruses"],"prefix":"10.1111","volume":"20","author":[{"ORCID":"https:\/\/orcid.org\/0009-0005-7925-7793","authenticated-orcid":false,"given":"Yang","family":"Fang","sequence":"first","affiliation":[{"name":"National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Disease, Chinese National Influenza Center National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention (Chinese Academy of Preventive Medicine)  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lei","family":"Yang","sequence":"additional","affiliation":[{"name":"National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Disease, Chinese National Influenza Center National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention (Chinese Academy of Preventive Medicine)  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shumei","family":"Zou","sequence":"additional","affiliation":[{"name":"National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Disease, Chinese National Influenza Center National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention (Chinese Academy of Preventive Medicine)  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liqi","family":"Liu","sequence":"additional","affiliation":[{"name":"National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Disease, Chinese National Influenza Center National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention (Chinese Academy of Preventive Medicine)  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenfei","family":"Zhu","sequence":"additional","affiliation":[{"name":"National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Disease, Chinese National Influenza Center National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention (Chinese Academy of Preventive Medicine)  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dayan","family":"Wang","sequence":"additional","affiliation":[{"name":"National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Disease, Chinese National Influenza Center National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention (Chinese Academy of Preventive Medicine)  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,7,24]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2025.01.019"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.adq0900"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/emi.2016.71"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2015.02.005"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.01370-23"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.3390\/vetsci12050447"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMc2415890"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1128\/mbio.00731-25"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-018-03663-5"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-024-08054-z"},{"key":"e_1_2_7_12_1","article-title":"Loss of \u03b12, 3\u2010Linked Sialoside in the Receptor\u2010Binding Site of a H5N1 Influenza Hemagglutinin Identified in a Human Patient","author":"Ni J. H.","year":"2026","journal-title":"bioRxiv"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2026.110949"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1080\/22221751.2026.2645856"}],"container-title":["Influenza and Other Respiratory Viruses"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/irv.70298","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/irv.70298","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/irv.70298","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,12]],"date-time":"2026-08-12T13:40:25Z","timestamp":1786542025000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/irv.70298"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,7]]},"references-count":13,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2026,7]]}},"alternative-id":["10.1111\/irv.70298"],"URL":"https:\/\/doi.org\/10.1111\/irv.70298","archive":["Portico"],"relation":{},"ISSN":["1750-2640","1750-2659"],"issn-type":[{"value":"1750-2640","type":"print"},{"value":"1750-2659","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,7]]},"assertion":[{"value":"2026-02-12","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-07-07","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-07-24","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70298"}}