{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T04:29:40Z","timestamp":1787632180501,"version":"build-2736575974"},"reference-count":40,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T00:00:00Z","timestamp":1787097600000},"content-version":"vor","delay-in-days":18,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T00:00:00Z","timestamp":1785542400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000865","name":"Bill and Melinda Gates Foundation","doi-asserted-by":"publisher","award":["OPP1211806"],"award-info":[{"award-number":["OPP1211806"]}],"id":[{"id":"10.13039\/100000865","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Influenza Resp Viruses"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Genomic surveillance can guide early detection of and response to emerging epidemics. Metatranscriptomic sequencing was used to investigate sub\u2010national respiratory virus circulation in Cambodia from 2020 to 2023.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Nasopharyngeal swabs were collected from individuals aged 2\u2009months to 65\u2009years with influenza\u2010like illness in four Cambodian hospitals. Metatranscriptomic data were generated by short\u2010read RNA sequencing. Bernoulli space\u2013time scan statistics were used to identify temporal virus clusters. Bayesian inference of phylogenetic trees was used to compute divergence times for temporally clustered, highly represented viruses (influenza A\/H3N2 and B,\n                      <jats:italic>Betacoronavirus 1<\/jats:italic>\n                      , respiratory syncytial virus [RSV] A and B), and publicly available global influenza virus genomes.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Of 1093 individuals, 499 (45.7%) had detectable respiratory viruses belonging to 68 distinct species. Moderate (\n                      <jats:italic>N<\/jats:italic>\n                      \u2009&gt;\u200920) discrete time\u2010clusters were noted of RSV\u2010A (37 cases),\n                      <jats:italic>Betacoronavirus 1<\/jats:italic>\n                      (21 cases), RSV\u2010B (22 cases), and A\/H3N2 (30 cases). The posterior median of time to most recent common ancestor ranged from 0.71\u2009years (95% HPD 0.38\u20131.10) for\n                      <jats:italic>Betacoronavirus 1<\/jats:italic>\n                      and 1.31\u2009years (95% HPD 0.60\u20133.20) for A\/H3N2, to 2.75\u2009years (1.82\u20134.26) for RSV\u2010A and 4.79\u2009years (2.39\u20137.74) for RSV\u2010B. A\/H3N2 and influenza B virus genomes mapped to clades 3C.2a1b.2a.2a and Victoria 1A.3a.2, respectively, and inter\u2010mixed with concurrent global strains.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Multiple respiratory viruses circulated at a sub\u2010national level in Cambodia from 2020 to 2023 despite pandemic disruptions. Influenza virus population diversity decreased during the height of lockdown but recovered in mid\u20102022. Re\u2010emerging influenza strains were distinct from historically circulating strains and clustered with contemporaneous global variants, suggesting multiple external introductions.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/irv.70306","type":"journal-article","created":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T03:11:04Z","timestamp":1787195464000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Mapping Sub\u2010National Respiratory Virus Circulation in Cambodia Using Metatranscriptomic Sequencing: A Multi\u2010Center Hospital\u2010Based Surveillance Study"],"prefix":"10.1111","volume":"20","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8293-3901","authenticated-orcid":false,"given":"Christina","family":"Yek","sequence":"first","affiliation":[{"name":"International Center of Excellence in Research Cambodia  Phnom Penh Cambodia"},{"name":"Laboratory of Clinical Immunology and 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