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We evaluated hybrid\u2010capture sequencing for HPIV genomic surveillance in C\u00f4te d'Ivoire using respiratory samples collected through the national sentinel surveillance network from January 2022 to December 2024.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Among 268 RT\u2010qPCR\u2010positive samples, 49 were selected for sequencing using the Twist Respiratory Virus Research hybrid\u2010capture panel on the Illumina iSeq 100 platform. Genome assembly and phylogenetic analyses were performed using CZ\u2010ID and IQ\u2010TREE.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Hybrid\u2010capture sequencing recovered 29 near\u2010complete genomes: 9 HPIV1, 10 HPIV2, and 10 HPIV3. Genome recovery was strongly dependent on viral load, with a marked negative correlation between cycle threshold (Ct) value and genome coverage (\n                      <jats:italic>r<\/jats:italic>\n                      \u2009=\u2009\u22120.91) and optimal performance at Ct\u2009\u2264\u200930. Viral co\u2010detections were identified in 25 sequenced samples; adenovirus type B was the most frequent co\u2010detected pathogen, while HPIV3 showed the broadest co\u2010detection profile. Phylogenetic analysis revealed limited diversity for HPIV1, greater diversity for HPIV2, and pronounced heterogeneity for HPIV3, including co\u2010circulation of divergent lineages.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Hybrid\u2010capture sequencing provides a practical approach for HPIV genomic surveillance in routine settings and offers detailed insight into HPIV circulation in West Africa, supporting lineage tracking and the design of future genomic surveillance strategies.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/irv.70335","type":"journal-article","created":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T08:51:02Z","timestamp":1791967862000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Advancing Genomic Surveillance of Human Parainfluenza Viruses in C\u00f4te d'Ivoire Using Twist Hybrid\u2010Capture Sequencing"],"prefix":"10.1111","volume":"20","author":[{"given":"Olivia Cariolh","family":"Koumba\u2010Koumba","sequence":"first","affiliation":[{"name":"Department of Epidemic Viruses Institut Pasteur de C\u00f4te d'Ivoire  Abidjan C\u00f4te d'Ivoire"},{"name":"UFR of Medical Sciences Universit\u00e9 F\u00e9lix Houphou\u00ebt\u2010Boigny  Abidjan C\u00f4te d'Ivoire"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9354-8689","authenticated-orcid":false,"given":"Yakoura Karidja","family":"Ouattara","sequence":"additional","affiliation":[{"name":"Department of Epidemic Viruses Institut Pasteur de C\u00f4te d'Ivoire  Abidjan C\u00f4te d'Ivoire"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5072-1805","authenticated-orcid":false,"given":"Herve A.","family":"Kadjo","sequence":"additional","affiliation":[{"name":"Department of Epidemic 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