{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,5]],"date-time":"2026-09-05T03:18:22Z","timestamp":1788578302656,"version":"build-2803163510"},"reference-count":42,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2013,3,21]],"date-time":"2013-03-21T00:00:00Z","timestamp":1363824000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["International Wound Journal"],"published-print":{"date-parts":[[2015,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Wound healing requires a proper functioning of keratinocytes that migrate, proliferate and lead to a competent wound closure. Impaired wound healing might be due to a disturbed keratinocyte function caused by the wound environment. Basically, chronic wound fluid (CWF) differs from acute wound fluid (AWF). The aim of this study was to analyse the effects of AWF and CWF on keratinocyte function. We therefore investigated keratinocyte migration and proliferation under the influence of AWF and CWF using\n                    <jats:styled-content style=\"fixed-case\">MTT<\/jats:styled-content>\n                    [3\u2010(4,5\u2010dimethylthiazol\u20102\u2010yl)\u20102,5\u2010diphenyltetrazolium bromide] test and scratch assay. We further measured the gene expression by\n                    <jats:styled-content style=\"fixed-case\">qRT\u2010PCR<\/jats:styled-content>\n                    regarding growth factors and matrixmetalloproteinases (MMPs) involved in regeneration processes. AWF had a positive impact on keratinocyte proliferation over time, whereas CWF had an anti\u2010proliferative effect. Keratinocyte migration was significantly impaired by CWF in contrast to an undisturbed wound closure under the influence of AWF.\n                    <jats:styled-content style=\"fixed-case\">MMP\u20109<\/jats:styled-content>\n                    expression was strongly upregulated by CWF compared with AWF. Keratinocyte function was significantly impaired by CWF. An excessive induction of\n                    <jats:styled-content style=\"fixed-case\">MMP\u20109<\/jats:styled-content>\n                    by CWF might lead to a permanent degradation of extracellular matrix and thereby prevent wounds from healing.\n                  <\/jats:p>","DOI":"10.1111\/iwj.12069","type":"journal-article","created":{"date-parts":[[2013,3,21]],"date-time":"2013-03-21T04:35:21Z","timestamp":1363840521000},"page":"143-149","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":30,"title":["Acute and chronic wound fluids influence keratinocyte function differently"],"prefix":"10.1111","volume":"12","author":[{"given":"Oliver C","family":"Thamm","sequence":"first","affiliation":[{"name":"Clinic of Plastic and Reconstructive Surgery, Handsurgery, Burn Care Center University of Witten\/Herdecke, Cologne\u2010Merheim Medical Center  Cologne Germany"},{"name":"Institute for Research in Operative Medicine 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