{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T10:28:47Z","timestamp":1781087327258,"version":"3.54.1"},"reference-count":33,"publisher":"Wiley","issue":"s1","license":[{"start":{"date-parts":[[2013,11,20]],"date-time":"2013-11-20T00:00:00Z","timestamp":1384905600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["International Wound Journal"],"published-print":{"date-parts":[[2013,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Negative pressure wound therapy with instillation (<jats:styled-content style=\"fixed-case\">NPWTi<\/jats:styled-content>) is increasingly used as an adjunct therapy for a wide variety of infected wounds. However, the effect of <jats:styled-content style=\"fixed-case\">NPWTi<\/jats:styled-content> on mature biofilm in wounds has not been determined. This study assessed the effects of <jats:styled-content style=\"fixed-case\">NPWTi<\/jats:styled-content> using saline or various antimicrobial solutions on mature <jats:italic>Pseudomonas aeruginosa<\/jats:italic> biofilm using an ex vivo porcine skin explant biofilm model. Treatment consisted of six\u2009cycles with 10\u2010minute exposure to instillation solution followed by 4\u2009hours of negative pressure at \u2212125\u2009mm Hg over a 24\u2010hour period. <jats:styled-content style=\"fixed-case\">NPWTi<\/jats:styled-content> using saline reduced bacterial levels by 1\u2010log (logarithmic) of 7\u2010log total colony\u2010forming units (<jats:styled-content style=\"fixed-case\">CFUs<\/jats:styled-content>). In contrast, instillation of 1% povidone iodine (2\u2010log), L\u2010solution (3\u2010log), 0\u00b705% chlorhexidine gluconate (3\u2010log), 0\u00b71% polyhexamethylene biguanide (4\u2010log), 0\u00b72% polydiallyldimethylammonium chloride (4\u2010log) and 10% povidone iodine (5\u2010log), all significantly reduced (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090\u00b7001) total <jats:styled-content style=\"fixed-case\">CFUs<\/jats:styled-content>. Scanning electron micrographs showed disrupted exopolymeric matrix of biofilms and damaged bacterial cells that correlated with <jats:styled-content style=\"fixed-case\">CFU<\/jats:styled-content> levels. Compared with previous studies assessing microbicidal effects of topical antimicrobial dressings on biofilms cultured on porcine skin explants, these ex vivo model data suggest that <jats:styled-content style=\"fixed-case\">NPWTi<\/jats:styled-content> with delivery of active antimicrobial agents enhances the reduction of <jats:styled-content style=\"fixed-case\">CFUs<\/jats:styled-content> by increasing destruction and removal of biofilm bacteria. These results must be confirmed in human studies.<\/jats:p>","DOI":"10.1111\/iwj.12180","type":"journal-article","created":{"date-parts":[[2013,11,20]],"date-time":"2013-11-20T05:57:18Z","timestamp":1384927038000},"page":"48-55","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":67,"title":["The effect of negative pressure wound therapy with periodic instillation using antimicrobial solutions on <i>Pseudomonas aeruginosa<\/i> biofilm on porcine skin explants"],"prefix":"10.1111","volume":"10","author":[{"given":"Priscilla L","family":"Phillips","sequence":"first","affiliation":[{"name":"Institute of Wound Research, Department of Obstetrics and Gynecology University of Florida Gainesville FL USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qingping","family":"Yang","sequence":"additional","affiliation":[{"name":"Institute of Wound Research, Department of Obstetrics and Gynecology University of Florida Gainesville FL USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gregory S","family":"Schultz","sequence":"additional","affiliation":[{"name":"Institute of Wound Research, Department of Obstetrics and Gynecology University of Florida Gainesville FL USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2013,11,20]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1097\/PRS.0b013e318200a427"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1742-481X.2009.00628.x"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.7205\/MILMED-D-09-00075"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1524-475X.2008.00390.x"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1742-481X.2012.00940.x"},{"issue":"1","key":"e_1_2_6_7_1","first-page":"4","article-title":"The history of the vac instill (r) therapy","volume":"37","author":"Fleischmann W","year":"2009","journal-title":"Infection"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1742-481X.2007.00423.x"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1177\/1534734604271810"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1097\/00001432-200404000-00004"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1524-475X.2007.00321.x"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-695X.2012.00968.x"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.284.5418.1318"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.15.2.167-193.2002"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-4362.1999.00738.x"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.42.8.3549-3557.2004"},{"key":"e_1_2_6_17_1","first-page":"299","volume-title":"Advances in wound care","author":"Phillips PL","year":"2010"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1097\/TA.0b013e318227ac5f"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0020-1383(11)00041-6"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1742-481X.2012.01014.x"},{"issue":"1","key":"e_1_2_6_21_1","first-page":"7","article-title":"Vac instill (r) treatment in severe septic abscess forming and perforating Ludwig's angina with large abscess formation in the neck and lower jawbone osteomyelitis due to dental caries with tooth root abscess\u00a0\u2013\u00a0a case report","volume":"37","author":"Wiedeck C","year":"2009","journal-title":"Infection"},{"key":"e_1_2_6_22_1","first-page":"37","article-title":"Combination of subatmospheric pressure dressing and gravity feed antibiotic instillation in the treatment of post\u2010surgical diabetic foot wounds: a case series","volume":"17","author":"Bernstein BH","year":"2005","journal-title":"Wounds"},{"issue":"1","key":"e_1_2_6_23_1","first-page":"11","article-title":"Advanced wound care with stable, super\u2010oxidized water\u00a0\u2013\u00a0a look at how combination therapy can optimize wound healing","volume":"18","author":"Wolvos TA","year":"2006","journal-title":"Wounds"},{"issue":"1","key":"e_1_2_6_24_1","first-page":"41","article-title":"The application of instillation combined with vacuum therapy in visceral surgery","volume":"37","author":"Reith HB","year":"2009","journal-title":"Infection"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00113-007-1360-1"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1524-475X.2009.00458.x"},{"issue":"1","key":"e_1_2_6_27_1","first-page":"5","article-title":"Therapy options with vac instill (r) in the treatment of open, superinfected injuries of the lower limps\u00a0\u2013\u00a0the erlangen algorithm","volume":"37","author":"Brem MH","year":"2009","journal-title":"Infection"},{"issue":"1","key":"e_1_2_6_28_1","first-page":"13","article-title":"Application of vac instill (r) therapy in case of periprosthetic infection in hip arthroplasty","volume":"37","author":"Lehner B","year":"2009","journal-title":"Infection"},{"issue":"1","key":"e_1_2_6_29_1","first-page":"18","article-title":"Management of early periprosthetic infections in the knee using the vacuum\u2010instillation therapy","volume":"37","author":"Koster G","year":"2009","journal-title":"Infection"},{"issue":"1","key":"e_1_2_6_30_1","first-page":"31","article-title":"The impact of vac instill (r) in severe soft tissue infections and necrotizing fasciitis","volume":"37","author":"Schintler MV","year":"2009","journal-title":"Infection"},{"issue":"1","key":"e_1_2_6_31_1","first-page":"33","article-title":"Vacuum\u2010assisted closure and instillation dressing (vac instill (r)) in the treatment of open fractures","volume":"37","author":"Amtsberg G","year":"2009","journal-title":"Infection"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.280.5361.295"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.mi.49.100195.003431"},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.1183\/09031936.94.07101746"}],"container-title":["International Wound Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fiwj.12180","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/iwj.12180","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,22]],"date-time":"2023-11-22T13:45:16Z","timestamp":1700660716000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/iwj.12180"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,11,20]]},"references-count":33,"journal-issue":{"issue":"s1","published-print":{"date-parts":[[2013,12]]}},"alternative-id":["10.1111\/iwj.12180"],"URL":"https:\/\/doi.org\/10.1111\/iwj.12180","archive":["Portico"],"relation":{},"ISSN":["1742-4801","1742-481X"],"issn-type":[{"value":"1742-4801","type":"print"},{"value":"1742-481X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,11,20]]},"assertion":[{"value":"2013-11-20","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}