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At present, silver nanoparticles (AgNPs) are reemerging as a viable topical treatment option for infections encountered in burns, open wounds and chronic ulcers. This study evaluated the in vitro mechanisms of two different sizes of AgNPs (4\u00b77 and 42 nm) toxicity in normal human dermal fibroblasts. The toxicity was evaluated by observing cell viability and oxidative stress parameters. In all toxicity endpoints studied (<jats:styled-content style=\"fixed-case\">MTT<\/jats:styled-content> and lactate dehydrogenase assays), AgNPs of 4\u00b77 nm were much more toxic than the large AgNPs (42 nm). The cytotoxicity of both AgNPs was greatly decreased by pre\u2010treatment with the antioxidant N\u2010acetyl\u2010L\u2010cysteine. The oxidative stress parameters showed significant increase in reactive oxygen species levels, depletion of glutathione level and slight, but not statistically significant inactivation of superoxide dismutase, suggesting generation of oxidative stress. Thus, AgNPs should be used with caution for the topical treatment of burns and wounds, medical devices etc, because their toxicity depends on the size, the smaller NPs being much more cytotoxic than the large.<\/jats:p>","DOI":"10.1111\/iwj.12244","type":"journal-article","created":{"date-parts":[[2014,2,26]],"date-time":"2014-02-26T02:04:38Z","timestamp":1393380278000},"page":"101-109","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":73,"title":["Interactions of manufactured silver nanoparticles of different sizes with normal human dermal fibroblasts"],"prefix":"10.1111","volume":"13","author":[{"given":"Alicia","family":"Avalos","sequence":"first","affiliation":[{"name":"Departamento de Nutrici\u00f3n, Bromatolog\u00eda y Tecnolog\u00eda de los Alimentos, Facultad de Veterinaria Universidad Complutense de Madrid  Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana 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