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Primary dermal fibroblasts were isolated from cultures of hypertrophic scars. Cells were cultured after addition of serum\u2010free Dulbecco's modified Eagle's medium supplemented with 5% (wt\/vol) PRP or platelet\u2010poor plasma (PPP). At 1, 4, 6, 8, 11, and 13 days after addition of PRP or PPP, the TGF\u2010\u03b21 and CTGF levels in supernatants were determined using solid\u2010phase enzyme\u2010linked immunosorbent assays. Quantitative reverse transcription polymerase chain reactions were performed to quantify TGF\u2010\u03b21 and CTGF mRNA expression levels. TGF\u2010\u03b21 mRNA expression in the PRP groups was lower than in the PPP groups from 4 to 13 days of culture, and there was statistically significant difference (<jats:italic>P<\/jats:italic> &lt; .01). CTGF level and mRNA expression in the PRP groups was lower than in the PPP groups, and there were statistically significant differences (<jats:italic>P<\/jats:italic> &lt; .01). Although further experiments will focus on clarifying the second messenger of the TGF\u2010\u03b21 negative feedback mechanism, the in vitro data presented show that PRP can potentially reduce <jats:italic>CTGF<\/jats:italic> and <jats:italic>CTGF<\/jats:italic> gene transcription by triggering the TGF\u2010\u03b21 signalling negative feedback mechanism.<\/jats:p>","DOI":"10.1111\/iwj.12896","type":"journal-article","created":{"date-parts":[[2018,5,21]],"date-time":"2018-05-21T08:50:49Z","timestamp":1526892649000},"page":"547-554","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":25,"title":["The effects of platelet\u2010rich plasma on hypertrophic scars fibroblasts"],"prefix":"10.1111","volume":"15","author":[{"given":"Seung Min","family":"Nam","sequence":"first","affiliation":[{"name":"Department of Plastic and Reconstructive Surgery, College of Medicine Soonchunhyang University  Bucheon Republic of 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