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We evaluated the NaPy pre\u2010treatment effect on permeability changes in the cell's plasma membrane (PM) following application of in vitro damaging\u2010level strains. Fibroblasts or myoblasts, respectively, models for superficial or deep\u2010tissue damage were grown in 0 or 1\u2009mM NaPy, emulating typical physiological or cell culture conditions. Cells were pre\u2010treated for 4 hours with 0 to 5\u2009mM NaPy prior to 3\u2010hour sustained, damaging\u2010level loads (12% strain). PM permeability was quantified by the cell uptake of small (4 kDa), fluorescent dextran compared with unstrained control using fluorescence\u2010activated cell sorting (FACS). Pre\u2010treatment with 1\u2009mM, and especially 5\u2009mM, NaPy significantly reduces damage to PM integrity. Long\u2010term NaPy pre\u2010exposure can improve protective treatment, affecting fibroblasts and myoblasts differently. Pre\u2010treating with NaPy, a natural cell metabolite, allows cells under damaging\u2010level mechanical loads to maintain their PM integrity, that is, to avoid loss of homeostasis and inevitable, eventual cell death, by preventing initial, microscale stages of PU formation. This pre\u2010treatment may be applied prior to planned periods of immobility, for example, planned surgery or transport, to prolong safe time in a position by preventing initial cell damage that can cascade and lead to PU formation.<\/jats:p>","DOI":"10.1111\/iwj.13173","type":"journal-article","created":{"date-parts":[[2019,8,13]],"date-time":"2019-08-13T00:59:37Z","timestamp":1565657977000},"page":"1153-1163","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Sodium pyruvate pre\u2010treatment prevents cell death due to localised, damaging mechanical strains in the context of pressure ulcers"],"prefix":"10.1111","volume":"16","author":[{"given":"Martha B.","family":"Alvarez\u2010Elizondo","sequence":"first","affiliation":[{"name":"Faculty of Biomedical Engineering Technion\u2010Israel Institute of Technology  Haifa 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