{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,14]],"date-time":"2026-01-14T15:01:53Z","timestamp":1768402913997,"version":"3.49.0"},"reference-count":46,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2019,10,27]],"date-time":"2019-10-27T00:00:00Z","timestamp":1572134400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["International Wound Journal"],"published-print":{"date-parts":[[2020,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>An altered balance between metalloproteinases (MMPs) and their inhibitor tissue inhibitor of metalloproteinases (TIMPs) may influence the healing process of a minor amputation following a successful vein graft. To speed up this process, negative pressure wound therapy (NPWT) and advanced moist wound dressing have been proposed. We determined the systemic and local release of MMP\u20101, \u20102, \u20103, \u20109, TIMP\u20101, and TIMP\u20102 by enzyme linked immunosorbent assay (ELISA) technique and their influences in the healing process in 26 patients who underwent minor amputation after a successful revascularisation procedure. Twelve patients (group 1) were medicated with NPWT and 14 (group 2) with advanced moist wound dressing. Plasma samples were collected on the morning of surgery and thereafter at 1, 3, and 5 months; exudates were collected 3\u2009days after surgery when amputation was performed and thereafter at 1, 3, and 5 months. Fifteen age\u2010matched healthy male volunteers served as controls. All wounds healed in 5 \u00b1\u20090.5 months. Follow\u2010up plasma and local release of MMP\u20101, \u20102, \u20103, and \u20109 were overall significantly lower when compared with the preoperative levels, while those of TIMP\u20101 and \u20102 were significantly higher with no differences among the groups. Despite no differences in the healing process being observed among the two types of medications, at 1 month the local release of MMP\u20102 and \u20109 was significantly lower (<jats:italic>P<\/jats:italic> =\u2009.013 and .047, respectively) and that of TIMP\u20101 was significantly higher (<jats:italic>P<\/jats:italic> =\u2009.042) in group 1 as compared to group 2. A correct and aggressive local approach to the wound is able to promote the healing of the lesion stimulating the extracellular matrix turnover with local MMP\/TIMP adequate balance and favouring the creation of granulation tissue. However, a successful restoration of an adequate blood flow remains the key point of a durable and rapid wound healing.<\/jats:p>","DOI":"10.1111\/iwj.13249","type":"journal-article","created":{"date-parts":[[2019,10,28]],"date-time":"2019-10-28T01:53:34Z","timestamp":1572227614000},"page":"149-157","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Local release of metalloproteinases and their inhibitors after a successful revascularisation procedure"],"prefix":"10.1111","volume":"17","author":[{"given":"Raffaele","family":"Grande","sequence":"first","affiliation":[{"name":"Department of Surgery \u201cPietro Valdoni\u201d \u201cSapienza\u201d University of Rome  Rome Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gioia","family":"Brachini","sequence":"additional","affiliation":[{"name":"Department of Surgery \u201cPietro Valdoni\u201d \u201cSapienza\u201d University of Rome  Rome 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