{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,4]],"date-time":"2025-11-04T10:58:51Z","timestamp":1762253931766,"version":"3.40.5"},"reference-count":33,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2021,9,23]],"date-time":"2021-09-23T00:00:00Z","timestamp":1632355200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["International Wound Journal"],"published-print":{"date-parts":[[2022,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Mechanical force plays a pivotal role in the pathogenesis of hypertrophic scar (HTS). Dermal fibroblasts and myofibroblasts are the key cells involved in HTS. Myofibroblasts in HTS possess different biochemical and biophysical characteristics by which myofibroblasts are often distinguished from fibroblasts. The role of mechanotransducers outside the nucleus in the pathogenesis of HTS has been reported in many studies. However, the role of Nesprin\u20102 in HTS is not clear. Hence, we aim to construct a cell model of HTS and explore the role of Nesprin\u20102 in this process. Myofibroblasts and fibroblasts were isolated from HTS and healthy skin tissues of the same patient. Fibroblasts were exposed to cyclic stretch with 10% magnitude and a frequency of 0.1\u00a0Hz for 3\u2009days, 5\u2009days, and 7\u2009days, respectively. After the cell model was confirmed, fibroblasts transfected with siRNA targeting human Nesprin\u20102 were exposed to cyclic stretch. The mechanical behaviour and biochemical reaction of the dermal fibroblasts were analysed. The stretched fibroblasts at day 5 showed the same mechanotransductive and biochemical features as unstretched myofibroblasts. Mechanical strain could induce the myofibroblasts differentiation and a cell model of HTS was established successfully at day 5. The expressions of lamin A\/C, alpha\u2010smooth muscle actin, transforming growth factor beta 1, and collagen type I in fibroblasts were reduced by the silencing of Nesprin\u20102. Mechanical strain could induce the myofibroblasts differentiation and silencing of Nesprin\u20102 could block the mechanical stimulation of terminal myofibroblasts differentiation. Nesprin\u20102 might be a potential target to treat the HTS.<\/jats:p>","DOI":"10.1111\/iwj.13694","type":"journal-article","created":{"date-parts":[[2021,9,24]],"date-time":"2021-09-24T01:19:19Z","timestamp":1632446359000},"page":"978-986","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Silencing of Nesprin\u20102 inhibits the differentiation of myofibroblasts from fibroblasts induced by mechanical stretch"],"prefix":"10.1111","volume":"19","author":[{"given":"Quanchen","family":"Xu","sequence":"first","affiliation":[{"name":"Department of Stomatology the Affiliated Hospital of Qingdao University  Qingdao China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuanxin","family":"Miao","sequence":"additional","affiliation":[{"name":"Department of Burn and Plastic Surgery the Affiliated Hospital of Qingdao University  Qingdao 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