{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,2]],"date-time":"2026-03-02T19:59:05Z","timestamp":1772481545927,"version":"3.50.1"},"reference-count":42,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2022,8,29]],"date-time":"2022-08-29T00:00:00Z","timestamp":1661731200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["International Wound Journal"],"published-print":{"date-parts":[[2023,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The diagnostic value of next\u2010generation sequencing (NGS) of blood samples from patients with periprosthetic joint infection (PJI) after total hip arthroplasty (THA) was evaluated by comparing it with drainage fluid NGS and bacterial culture. The study was designed as a retrospective diagnostic test. Thirty\u2010six infected patients were diagnosed with PJI according to the Musculoskeletal Infection Society (MSIS) criteria and 57 volunteers were included in our study. NGS and bacterial culture were chosen to detect PJI after THA. Blood samples and drainage fluid were collected for NGS, and the drainage fluid, which was collected at the same time as the NGS drainage fluid sample, was used for bacterial culture. The primary outcomes of interest were sensitivity, specificity, and accuracy. In the infection group, 31 patients showed positive results by blood sample NGS, 33 patients showed positive results by drainage fluid NGS, and 17 patients showed positive bacterial culture results. In the control group, the results of 2 blood sample NGS, 16 drainage fluid NGS, and 3 bacterial cultures were positive. The sensitivity, specificity, and accuracy of the blood sample were 0.86, 0.96, and 0.92, respectively. The sensitivity, specificity, and accuracy of the drainage fluid samples were 0.92, 0.72, and 0.80, respectively. The sensitivity, specificity, and accuracy of bacterial culture were 0.47, 0.95, and 0.79, respectively. The study demonstrated that both the sensitivity and specificity of NGS were higher than those of bacterial culture, regardless of the kind of sample. Compared with drainage fluid NGS, the sensitivity of blood sample NGS was slightly lower (0.86 vs 0.92), but blood sample NGS showed higher specificity (0.96 vs 0.72). In total, the diagnostic value of blood sample NGS was superior to that of drainage fluid NGS and bacterial culture. The majority of infected patients could be identified by blood sample NGS. Moreover, because of its high specificity, blood sample NGS can not only detect infectious bacteria but also distinguish infectious from non\u2010infectious bacteria, which is dramatically different from using drainage fluid NGS.<\/jats:p>","DOI":"10.1111\/iwj.13943","type":"journal-article","created":{"date-parts":[[2022,8,30]],"date-time":"2022-08-30T02:23:30Z","timestamp":1661826210000},"page":"961-970","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["The diagnostic value of blood sample <scp>NGS<\/scp> in patients with early periprosthetic joint infection after total hip arthroplasty"],"prefix":"10.1111","volume":"20","author":[{"given":"Bingshi","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of Osteonecrosis and Hip Surgery the Third Hospital of Hebei Medical University  Shijiazhuang Hebei PR China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mengnan","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Osteonecrosis and Hip Surgery the Third Hospital of Hebei Medical University  Shijiazhuang Hebei PR China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yawei","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Osteonecrosis and Hip Surgery the Third Hospital of Hebei Medical University  Shijiazhuang Hebei PR China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Binquan","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Osteonecrosis and Hip Surgery the Third Hospital of Hebei Medical University  Shijiazhuang Hebei PR China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zeming","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Osteonecrosis and Hip Surgery the Third Hospital of Hebei Medical University  Shijiazhuang Hebei PR China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiao","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Osteonecrosis and Hip Surgery the Third Hospital of Hebei Medical University  Shijiazhuang Hebei PR China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jia","family":"Huo","sequence":"additional","affiliation":[{"name":"Department of Osteonecrosis and Hip Surgery the Third Hospital of Hebei Medical University  Shijiazhuang Hebei PR China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yongtai","family":"Han","sequence":"additional","affiliation":[{"name":"Department of Osteonecrosis and Hip Surgery the Third Hospital of Hebei Medical University  Shijiazhuang Hebei PR China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2022,8,29]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10096-019-03520-3"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1302\/2058-5241.6.200099"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11999-014-4078-8"},{"key":"e_1_2_9_5_1","unstructured":"AkindolireJ MarshJ HowardJ LantingB SomervilleL VasarhelyiE.The economic impact of periprosthetic infection in total hip arthroplasty;2017."},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.idc.2017.01.008"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.2106\/JBJS.17.00123"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.5435\/JAAOS-D-17-00674"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.000958"},{"key":"e_1_2_9_10_1","article-title":"Diagnostic value of next\u2010generation sequencing to detect Periprosthetic joint infection","volume":"22","author":"Yin H","journal-title":"BMC Musculoskelet Disord"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00264-017-3430-5"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11999-013-3070-z"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.amjmed.2014.03.013"},{"key":"e_1_2_9_14_1","article-title":"Can metagenomic next\u2010generation sequencing identify the pathogens responsible for culture\u2010negative prosthetic joint infection?","volume":"20","author":"Wang C","year":"2020","journal-title":"BMC Infect Dis"},{"issue":"2","key":"e_1_2_9_15_1","first-page":"74","article-title":"Review of common pathogens in the BONE and BLOOD cultures and antibiotic treatment in patients with sternal osteomyelitis","volume":"156","author":"Benkler M","year":"2017","journal-title":"Harefuah"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijid.2020.07.039"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1515\/CCLM.2008.157"},{"key":"e_1_2_9_18_1","article-title":"Antisepsis for blood culture extraction. 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