{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T16:47:57Z","timestamp":1790182077616,"version":"4.1.0"},"reference-count":23,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2023,1,17]],"date-time":"2023-01-17T00:00:00Z","timestamp":1673913600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100007128","name":"Natural Science Foundation of Shaanxi Province","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100007128","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["International Wound Journal"],"published-print":{"date-parts":[[2023,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>To investigate the application value of next\u2010generation sequencing (NGS) technology in the detection of pathogenic bacteria in the periprosthetic joint infection after arthroplasty. Twenty\u2010two cases of patients with joint infection after arthroplasty in our hospital from March 2020 to March 2021 were selected, with 11 cases of knee and 11 cases of hip, including 8 cases of male and 14 cases of female, and an average age of 63.55\u2009\u00b1\u200913.11\u2009years old (range from 28 to 85). Microbiological culture results of synovial fluid and periprosthetic joint tissue and NGS results of periprosthetic joint tissue were collected. The detection rate of NGS and microbiological culture were calculated and statistically analysed by paired <jats:italic>\u03c7<\/jats:italic><jats:sup>2<\/jats:sup> test. Among the 22 patients with joint infection after arthroplasty, the positive rate of NGS was 90.91% (20\/22), whereas the positive rate of bacterial culture was 50.00% (11\/22). Paired chi\u2010square test showed a statistically significant difference in the detection rate between the two groups (<jats:italic>P<\/jats:italic>\u00a0=\u00a0.0029). In the detection of pathogenic microorganism, NGS detected 12 kinds of bacteria, <jats:italic>Staphylococcus aureus<\/jats:italic> in 3 patients, <jats:italic>Staphylococcus epidermidis<\/jats:italic> in 5 cases, <jats:italic>Streptococcus<\/jats:italic> 1 case, <jats:italic>Streptococcus dysgalactiae<\/jats:italic> 1 case, <jats:italic>Xanthomonas campestris<\/jats:italic> 3 cases, <jats:italic>Escherichia coli<\/jats:italic> 2 cases, <jats:italic>Bacillus cereus<\/jats:italic> 2 cases, <jats:italic>Klebsiella pneumoniae<\/jats:italic> 1 case, <jats:italic>Finegoldia magna<\/jats:italic> 1 case, <jats:italic>Corynebacterium klopensteriella<\/jats:italic> in 1 case, <jats:italic>Brucella<\/jats:italic> 1 case, and <jats:italic>Aspergillus flavus<\/jats:italic> 1 case. Bacterial culture detected 6 kinds of bacteria, included 5 cases of <jats:italic>Staphylococcus epidermis<\/jats:italic> (including 3 cases of Methicillin\u2010resistant coagulase\u2010negative Staphylococcus, (MRSCoN)), 2 cases of <jats:italic>Staphylococcus aureus<\/jats:italic> (both Methicillin\u2010resistant <jats:italic>Staphylococcus aureus<\/jats:italic>, (MRSA)), 1 case of <jats:italic>Klebsiella pneumoniae<\/jats:italic>, 1 case of <jats:italic>Staphylococcus hominis<\/jats:italic> (MRSCoN), 1 case of G<jats:sup>+<\/jats:sup> <jats:italic>bacillus<\/jats:italic>, and 1 case of <jats:italic>Brucella<\/jats:italic>. Compared with bacterial culture, NGS technology has some advantages in the detection efficiency, detection rate, and comprehensiveness, which might be greater diagnostic value in the joint fluid of infection after arthroplasty.<\/jats:p>","DOI":"10.1111\/iwj.14087","type":"journal-article","created":{"date-parts":[[2023,1,17]],"date-time":"2023-01-17T12:23:01Z","timestamp":1673958181000},"page":"2121-2128","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Application of next\u2010generation sequencing technology in the detection of pathogenic bacteria of the periprosthetic joint infection after arthroplasty"],"prefix":"10.1111","volume":"20","author":[{"given":"Yu","family":"Chang","sequence":"first","affiliation":[{"name":"Department of Clinical Pharmacy, Honghui Hospital Xi'an Jiaotong University  Xi'an People's Republic of 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