{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T15:34:36Z","timestamp":1759332876408,"version":"build-2065373602"},"reference-count":39,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2024,3,11]],"date-time":"2024-03-11T00:00:00Z","timestamp":1710115200000},"content-version":"vor","delay-in-days":10,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100007129","name":"Natural Science Foundation of Shandong Province","doi-asserted-by":"publisher","award":["ZR2020MH158","ZR2020QH182","ZR2022MH155"],"award-info":[{"award-number":["ZR2020MH158","ZR2020QH182","ZR2022MH155"]}],"id":[{"id":"10.13039\/501100007129","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82203760"],"award-info":[{"award-number":["82203760"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["International Wound Journal"],"published-print":{"date-parts":[[2024,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>This study aims to investigate the role of anoikis\u2010related genes in diabetic foot (DF) by utilizing bioinformatics analysis to identify key genes associated with anoikis in DF. We selected the GEO datasets GSE7014, GSE80178 and GSE68183 for the extraction and analysis of differentially expressed anoikis\u2010related genes (DE\u2010ARGs). GO analysis and KEGG analysis indicated that DE\u2010ARGs in DF were primarily enriched in apoptosis, positive regulation of MAPK cascade, anoikis, focal adhesion and the PI3K\u2010Akt signalling pathway. Based on the LASSO and SVM\u2010RFE algorithms, we identified six characteristic genes. ROC curve analysis revealed that these six characteristic genes had an area under the curve (AUC) greater than 0.7, indicating good diagnostic efficacy. Expression analysis in the validation set revealed downregulation of CALR in DF, consistent with the training set results. GSEA results demonstrated that CALR was mainly enriched in blood vessel morphogenesis, endothelial cell migration, ECM\u2010receptor interaction and focal adhesion. The HPA database revealed that CALR was moderately enriched in endothelial cells, and CALR was found to interact with 63 protein\u2010coding genes. Functional analysis with DAVID suggested that CALR and associated genes were enriched in the phagosome component. CALR shows promise as a potential marker for the development and treatment of DF.<\/jats:p>","DOI":"10.1111\/iwj.14771","type":"journal-article","created":{"date-parts":[[2024,3,12]],"date-time":"2024-03-12T01:12:25Z","timestamp":1710205945000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Identification and clinical validation of the role of anoikis\u2010related genes in diabetic foot"],"prefix":"10.1111","volume":"21","author":[{"given":"Nan","family":"Su","sequence":"first","affiliation":[{"name":"Department of Neurosurgery, Qilu Hospital, Cheeloo College of Medicine and Institute of Brain and Brain\u2010Inspired Science Shandong University  Jinan China"},{"name":"School of Medicine, Cheeloo College of Medicine Shandong University  Jinan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiwei","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery, Qilu 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