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For ease of cleaning and processing, these layers are often separated with or without re\u2010lamination and the trophoblast layer is typically discarded, both of which can negatively affect the abundance of native biological factors and make the grafts difficult to handle. Thus, a full\u2010thickness placental membrane that includes a fully\u2010intact decellularized trophoblast layer was developed for homologous clinical use as a protective barrier and scaffold in soft tissue repairs. Here, we demonstrate that this full\u2010thickness placental membrane is effectively decellularized while retaining native extracellular matrix (ECM) scaffold and biological factors, including the full trophoblast layer. Following processing, it is porous, biocompatible, supports cell proliferation in vitro, and retains its biomechanical strength and the ability to pass through a cannula without visible evidence of movement or damage. Finally, it was accepted as a natural scaffold in vivo with evidence of host\u2010cell infiltration, angiogenesis, tissue remodelling, and structural layer retention for up to 10\u2009weeks in a murine subcutaneous implant model.<\/jats:p>","DOI":"10.1111\/iwj.14888","type":"journal-article","created":{"date-parts":[[2024,4,30]],"date-time":"2024-04-30T08:22:17Z","timestamp":1714465337000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Characterization of a full\u2010thickness decellularized and lyophilized human placental membrane for clinical applications"],"prefix":"10.1111","volume":"21","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2723-5542","authenticated-orcid":false,"given":"Bradley","family":"Wetzell","sequence":"first","affiliation":[{"name":"Global Scientific Affairs and Clinical Engagement LifeNet Health\u00ae  Virginia Beach Virginia USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Britini","family":"Ork","sequence":"additional","affiliation":[{"name":"Institute of Regenerative Medicine LifeNet Health  Virginia Beach Virginia USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Davorka","family":"Softic","sequence":"additional","affiliation":[{"name":"Institute of Regenerative Medicine LifeNet Health  Virginia Beach Virginia USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jennifer","family":"Morse","sequence":"additional","affiliation":[{"name":"Institute of Regenerative Medicine LifeNet Health  Virginia Beach Virginia USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"William","family":"Hutchens","sequence":"additional","affiliation":[{"name":"Institute of Regenerative Medicine LifeNet Health  Virginia Beach Virginia USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fanwei","family":"Meng","sequence":"additional","affiliation":[{"name":"Institute of Regenerative Medicine LifeNet Health  Virginia Beach Virginia USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julie B.","family":"McLean","sequence":"additional","affiliation":[{"name":"Global Scientific Affairs and Clinical Engagement LifeNet Health\u00ae  Virginia Beach Virginia USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark A.","family":"Moore","sequence":"additional","affiliation":[{"name":"Global Scientific Affairs and Clinical Engagement LifeNet Health\u00ae  Virginia Beach Virginia USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaofei","family":"Qin","sequence":"additional","affiliation":[{"name":"Institute of Regenerative Medicine LifeNet Health  Virginia Beach Virginia USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,4,30]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-816413-6.00016-2"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cpm.2018.02.004"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.4103\/jispcd.JISPCD_359_16"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1136\/pgmj.38.438.193"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1177\/0363546515612750"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbm.b.33141"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/sctm.19-0101"},{"key":"e_1_2_9_9_1","unstructured":"QinX ChenS AschenbachL ChenJ.WO\/2017\/112934. 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