{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,21]],"date-time":"2026-08-21T12:28:50Z","timestamp":1787315330576,"version":"build-2736575974"},"reference-count":49,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2025,3,11]],"date-time":"2025-03-11T00:00:00Z","timestamp":1741651200000},"content-version":"vor","delay-in-days":10,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R21 AI175650\u201001A1"],"award-info":[{"award-number":["R21 AI175650\u201001A1"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000005","name":"U.S. Department of Defense","doi-asserted-by":"publisher","award":["MIDRP MI230044"],"award-info":[{"award-number":["MIDRP MI230044"]}],"id":[{"id":"10.13039\/100000005","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["International Wound Journal"],"published-print":{"date-parts":[[2025,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Biofilms complicate wound care by causing recurrent infections that are often resistant to debridement and are highly antibiotic\u2010tolerant. We investigated whether the addition of a biofilm dispersal agent could improve the efficacy of debridement. The previous studies have indicated that a glycoside hydrolase cocktail of alpha\u2010amylase and cellulase can act as a potent biofilm dispersal agent. With in\u00a0vitro and ex\u00a0vivo\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Pseudomonas aeruginosa<\/jats:italic>\n                    <\/jats:styled-content>\n                    biofilm models, we compared glycoside hydrolases against other, clinically relevant, enzymatic debridement agents (papain, bromelain, and collagenase). Glycoside hydrolase biofilm dispersal was dose\u2010dependent. However, at doses of 1% or above, glycoside hydrolases outperformed, or were comparable, to other enzymatic debridement agents. With our in\u00a0vivo surgical wound infection model, we evaluated biofilm dispersal using infection dissemination as a proxy. We found that sharp debridement followed by multiple glycoside hydrolase treatments enhanced biofilm dispersal. Furthermore, a single dose of glycoside hydrolase in combination with debridement decreased infection load in acute wounds. Similarly, when we treated established 5\u2010day\u2010old infections, we saw a decrease in infection load and no infection dissemination. Overall, our data suggest that debridement enhances the efficacy of a topical antibiotic ointment, allowing for greater infection clearance.\n                  <\/jats:p>","DOI":"10.1111\/iwj.70145","type":"journal-article","created":{"date-parts":[[2025,3,12]],"date-time":"2025-03-12T04:54:18Z","timestamp":1741755258000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Biofilm Dispersal and Wound Infection Clearance With Preclinical Debridement Agents"],"prefix":"10.1111","volume":"22","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0947-4977","authenticated-orcid":false,"given":"Rebecca Elizabeth","family":"Schneider","sequence":"first","affiliation":[{"name":"Department of Surgery Texas Tech University Health Sciences Center  Lubbock Texas USA"},{"name":"Department of Immunology and Molecular Microbiology Texas Tech University Health Sciences Center  Lubbock Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jason Valentino","family":"Hamdan","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Biochemistry Texas Tech University  Lubbock Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kendra Pauline","family":"Rumbaugh","sequence":"additional","affiliation":[{"name":"Department of Surgery Texas Tech University Health Sciences Center  Lubbock Texas USA"},{"name":"Department of Immunology and Molecular Microbiology Texas Tech University Health Sciences Center  Lubbock Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,3,11]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/iwj.12925"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/wrr.12994"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jgar.2021.05.010"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jccw.2015.08.003"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/iwj.12760"},{"key":"e_1_2_9_7_1","first-page":"1","article-title":"Debridement for Surgical Wounds","volume":"9","author":"Smith F.","year":"2013","journal-title":"Cochrane Database of Systematic Reviews"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.5114\/ada.2022.117572"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1089\/wound.2011.0333"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1309\/LMBNSWKUI4JPN7SO"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/iwj.12187"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.hansur.2016.06.001"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.12968\/jowc.2023.32.Sup3b.S4"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.202112218"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.12968\/jowc.2014.23.Sup9.S4"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.amsu.2021.102876"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jconrel.2022.05.013"},{"key":"e_1_2_9_18_1","first-page":"71","article-title":"Enzymatic Degradation of In\u00a0Vitro \n                     Staphylococcus aureus\n                   Biofilms Supplemented With Human Plasma","volume":"9","author":"Watters C. 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