{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T03:11:41Z","timestamp":1761016301670},"reference-count":35,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2004,5,14]],"date-time":"2004-05-14T00:00:00Z","timestamp":1084492800000},"content-version":"vor","delay-in-days":13,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Epilepsia"],"published-print":{"date-parts":[[2004,5]]},"abstract":"<jats:p><jats:bold>Summary:\u2003<\/jats:bold>  <jats:italic>Purpose:<\/jats:italic> Mossy fiber sprouting is a common abnormality found in patients and models of temporal lobe epilepsy. The role of mossy fiber sprouting in epileptogenesis is unclear, and its blockade would be useful experimentally and perhaps therapeutically. Results from previous attempts to block mossy fiber sprouting have been disappointing or controversial. In some brain regions, prolonged application of the sodium channel blocker tetrodotoxin prevents axon sprouting and posttrauma epileptogenesis. The present study tested the hypothesis that prolonged, focal infusion of tetrodotoxin would block mossy fiber sprouting after an epileptogenic treatment.<\/jats:p><jats:p> <jats:italic>Methods:<\/jats:italic> Adult rats were treated with pilocarpine to induce status epilepticus. Several hours to 3 days after pilocarpine treatment, a pump with a cannula directed toward the dentate gyrus was implanted to deliver 10 \u03bc<jats:italic>M<\/jats:italic> tetrodotoxin or vehicle alone at 0.25 \u03bcl\/h. This method blocks local EEG activity in the hippocampus (Galvan et al. <jats:italic>J Neurosci<\/jats:italic> 2000; 20:2904\u201316). After 28 days of continuous infusion, rats were perfused with fixative, and their hippocampi analyzed anatomically with stereologic techniques.<\/jats:p><jats:p> <jats:italic>Results:<\/jats:italic> Tetrodotoxin infusion was verified immunocytochemically in tetrodotoxin\u2010treated but not vehicle\u2010treated hippocampi. Tetrodotoxin\u2010infused and vehicle\u2010infused hippocampi displayed similar levels of hilar neuron loss. The Timm stain revealed mossy fiber sprouting regardless of whether hippocampi were treated with tetrodotoxin infusion, vehicle infusion, or neither.<\/jats:p><jats:p> <jats:italic>Conclusions:<\/jats:italic> Prolonged infusion of tetrodotoxin did not block mossy fiber sprouting. This finding suggests that sodium channel\u2013mediated neuronal activity is not necessary for mossy fiber sprouting after an epileptogenic treatment.<\/jats:p>","DOI":"10.1111\/j.0013-9580.2004.67103.x","type":"journal-article","created":{"date-parts":[[2004,5,14]],"date-time":"2004-05-14T13:35:55Z","timestamp":1084541755000},"page":"452-458","source":"Crossref","is-referenced-by-count":20,"title":["Prolonged Infusion of Tetrodotoxin Does Not Block Mossy Fiber Sprouting in Pilocarpine\u2010treated Rats"],"prefix":"10.1111","volume":"45","author":[{"given":"Paul S.","family":"Buckmaster","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2004,5,14]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(80)90825-2"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1002\/ana.410260303"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(89)90234-5"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.10-01-00267.1990"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/0306-4522(91)90380-7"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1528-1157.1995.tb02566.x"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.15-05-03990.1995"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1002\/cne.902000310"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.21-21-08523.2001"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1002\/ana.1230"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.2449733"},{"key":"e_1_2_6_13_2","doi-asserted-by":"crossref","first-page":"4622","DOI":"10.1523\/JNEUROSCI.13-11-04622.1993","article-title":"Developmental analysis of hippocampal mossy fiber outgrowth in a mutant mouse with inherited spike\u2010wave seizures","volume":"13","author":"Qiao X","year":"1993","journal-title":"J Neurosci"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/0306-4522(87)90237-5"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1528-1157.1993.tb02123.x"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1096-9861(19970901)385:3<385::AID-CNE4>3.0.CO;2-#"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0920-1211(99)00088-1"},{"key":"e_1_2_6_18_2","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.05-04-01016.1985"},{"key":"e_1_2_6_19_2","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.22-14-06052.2002"},{"key":"e_1_2_6_20_2","doi-asserted-by":"publisher","DOI":"10.1002\/ana.10490"},{"key":"e_1_2_6_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/0304-3940(92)90306-R"},{"key":"e_1_2_6_22_2","doi-asserted-by":"publisher","DOI":"10.1006\/exnr.1997.6553"},{"key":"e_1_2_6_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0304-3940(97)00267-X"},{"key":"e_1_2_6_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0920-1211(98)00049-7"},{"key":"e_1_2_6_25_2","doi-asserted-by":"publisher","DOI":"10.1152\/jn.2002.88.4.2075"},{"key":"e_1_2_6_26_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.7123262"},{"key":"e_1_2_6_27_2","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.22-14-06062.2002"},{"key":"e_1_2_6_28_2","doi-asserted-by":"publisher","DOI":"10.1002\/1531-8249(199908)46:2<234::AID-ANA13>3.0.CO;2-Q"},{"key":"e_1_2_6_29_2","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.20-08-02904.2000"},{"key":"e_1_2_6_30_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0041-0101(01)00179-9"},{"key":"e_1_2_6_31_2","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(95)00167-O"},{"key":"e_1_2_6_32_2","doi-asserted-by":"publisher","DOI":"10.1002\/hipo.1044"},{"key":"e_1_2_6_33_2","doi-asserted-by":"publisher","DOI":"10.1002\/ar.1092310411"},{"key":"e_1_2_6_34_2","doi-asserted-by":"publisher","DOI":"10.1152\/jn.2001.85.3.1067"},{"key":"e_1_2_6_35_2","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.15-12-08234.1995"},{"key":"e_1_2_6_36_2","first-page":"77","article-title":"Developmental mechanisms that generate precise patterns of neuronal connectivity","volume":"10","author":"Goodman CS","year":"1993","journal-title":"Neuron"}],"container-title":["Epilepsia"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.0013-9580.2004.67103.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.0013-9580.2004.67103.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,13]],"date-time":"2023-10-13T01:39:51Z","timestamp":1697161191000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.0013-9580.2004.67103.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2004,5]]},"references-count":35,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2004,5]]}},"alternative-id":["10.1111\/j.0013-9580.2004.67103.x"],"URL":"https:\/\/doi.org\/10.1111\/j.0013-9580.2004.67103.x","archive":["Portico"],"relation":{},"ISSN":["0013-9580","1528-1167"],"issn-type":[{"value":"0013-9580","type":"print"},{"value":"1528-1167","type":"electronic"}],"subject":[],"published":{"date-parts":[[2004,5]]}}}