{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,22]],"date-time":"2025-10-22T17:38:31Z","timestamp":1761154711508},"reference-count":38,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2004,1,13]],"date-time":"2004-01-13T00:00:00Z","timestamp":1073952000000},"content-version":"vor","delay-in-days":12,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Transfusion"],"published-print":{"date-parts":[[2004,1]]},"abstract":"<jats:p><jats:bold>BACKGROUND:\u2002<\/jats:bold> WBC depletion by filtration may prevent the transmission of HTLV\u2010I, which requires cell\u2010to\u2010cell contact. The removal of HTLV\u2010I\u2010infected cells in routinely filtered blood cell components was measured.<\/jats:p><jats:p><jats:bold>STUDY DESIGN AND METHODS:\u2002<\/jats:bold> The study was conducted in Martinique where systematic screening for HTLV\u2010I and \u2010II and universal leukoreduction are mandatory. HTLV\u2010I was quantified by use of real\u2010time PCR in 8 RBC units and 4 PLT concentrates before and after filtration. HTLV\u2010I proviral load in PBMNCs was determined in five of the eight HTLV\u2010I\u2010infected blood donors.<\/jats:p><jats:p><jats:bold>RESULTS:\u2002<\/jats:bold> The amount of MNC\u2010associated HTLV\u2010I DNA in RBC units before filtration was 21 \u00d7 10<jats:sup>6<\/jats:sup>\u00b1 29 \u00d7 10<jats:sup>6<\/jats:sup> copies (mean \u00b1 SD). HTLV\u2010I was detected in 4 of 8 RBC units after filtration, with a number of copies in the MNC fraction ranging from 20 to 140, following a 4.9 to 5.8 log reduction. Flow cytometry analysis performed in 2 of the filtered RBC units containing detectable HTLV\u2010I showed suboptimal and out\u2010of\u2010range leukoreduction (0.56 \u00d7 10<jats:sup>6<\/jats:sup> and 1.22 \u00d7 10<jats:sup>6<\/jats:sup> residual WBCs). HTLV was not detected in filtered RBCs from the blood donor with the highest percentage of HTLV\u2010I\u2010infected PBMCs (9%).<\/jats:p><jats:p><jats:bold>CONCLUSION:\u2002<\/jats:bold> This study confirms that HTLV\u2010I\u2010infected cells can be detected in filtered blood cell components and shows that optimal leukoreduction is critical for HTLV\u2010I removal.<\/jats:p>","DOI":"10.1111\/j.0041-1132.2004.00629.x","type":"journal-article","created":{"date-parts":[[2004,12,29]],"date-time":"2004-12-29T20:59:55Z","timestamp":1104353995000},"page":"42-48","source":"Crossref","is-referenced-by-count":20,"title":["Evaluation of HTLV\u2010I removal by filtration of blood cell components in a routine setting"],"prefix":"10.1111","volume":"44","author":[{"given":"Raymond","family":"C\u00e9saire","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Brigitte","family":"K\u00e9rob\u2010Bauchet","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Olivier","family":"Bourdonn\u00e9","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H\u00e9l\u00e8ne","family":"Maier","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Karim","family":"Ould Amar","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Philippe","family":"Halbout","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Axelle","family":"Deh\u00e9e","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nathalie","family":"D\u00e9sir\u00e9","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fabienne","family":"Dantin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Odile","family":"B\u00e9ra","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Agn\u00e8s","family":"L\u00e9zin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2004,1,13]]},"reference":[{"key":"e_1_2_5_2_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.78.10.6476"},{"key":"e_1_2_5_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(85)92734-5"},{"key":"e_1_2_5_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(86)91298-5"},{"key":"e_1_2_5_5_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1537-2995.1991.31191096189.x"},{"key":"e_1_2_5_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1047-2797(01)00231-9"},{"key":"e_1_2_5_7_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1423-0410.1984.tb00083.x"},{"key":"e_1_2_5_8_2","doi-asserted-by":"crossref","first-page":"2043","DOI":"10.1001\/archinte.1991.00400100115019","article-title":"Transmission of human T\u2010lymphotropic virus types I and II by blood transfusion: a retrospective study of recipients of blood components (1983 through 1988). 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