{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,22]],"date-time":"2025-10-22T17:36:38Z","timestamp":1761154598446},"reference-count":42,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2004,3,15]],"date-time":"2004-03-15T00:00:00Z","timestamp":1079308800000},"content-version":"vor","delay-in-days":43,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Vox Sanguinis"],"published-print":{"date-parts":[[2004,2]]},"abstract":"<jats:p><jats:bold>Background and Objectives\u2002<\/jats:bold> The risk of haemophiliacs contracting variant Creutzfeldt\u2010Jakob disease (vCJD) via treatment with factor VIII concentrates is not known. Therefore, in order to determine the extent to which the vCJD agent might be removed during the preparation of factor VIII concentrate, the partitioning of a bovine spongiform encephalopathy (BSE)\u2010derived agent was measured over the main purification step used to prepare the Scottish National Blood Transfusion Service high\u2010purity factor VIII concentrate (Liberate\u00ae).<\/jats:p><jats:p><jats:bold>Materials and Methods\u2002<\/jats:bold> Murine\u2010passaged BSE (strain 301V), in the form of a microsomal fraction prepared from infected brain, was used to \u2018spike\u2019 a solution of factor VIII of intermediate purity. The \u2018spiked\u2019 starting material was subjected to solvent\u2013detergent treatment and then to anion\u2010exchange chromatography with Toyopearl DEAE\u2010650M. All fractions were tested for 301V infectivity using a murine bioassay, including the procedures used to clean the ion\u2010exchange media after use.<\/jats:p><jats:p><jats:bold>Results\u2002<\/jats:bold> BSE 301V infectivity was reduced by 2\u00b79 log<jats:sub>10<\/jats:sub> in the fibrinogen fraction and by 2\u00b77 log<jats:sub>10<\/jats:sub> in the factor VIII fraction. Over 99% of the added 301V infectivity remained bound to the ion\u2010exchange column after elution of factor VIII. A large quantity of infectivity was subsequently removed by washing the ion\u2010exchange media with 2\u00a0<jats:sc>m<\/jats:sc> NaCl. No further BSE 301V infectivity was detected in column eluates after treatment with 0\u00b71\u00a0<jats:sc>m<\/jats:sc> NaOH or a second wash with 2\u00a0<jats:sc>m<\/jats:sc> NaCl.<\/jats:p><jats:p><jats:bold>Conclusions\u2002<\/jats:bold> Results using a BSE\u2010derived agent suggest that vCJD infectivity would be substantially removed by the ion\u2010exchange process used in the preparation of fibrinogen and factor VIII concentrate. Although 301V infectivity remained bound to the ion\u2010exchange matrix following elution of factor VIII, this appeared to be eliminated by the procedure used for cleaning the ion\u2010exchange media after each use.<\/jats:p>","DOI":"10.1111\/j.0042-9007.2004.00403.x","type":"journal-article","created":{"date-parts":[[2004,3,15]],"date-time":"2004-03-15T16:06:55Z","timestamp":1079366815000},"page":"92-99","source":"Crossref","is-referenced-by-count":30,"title":["Distribution of a bovine spongiform encephalopathy\u2010derived agent over ion\u2010exchange chromatography used in the preparation of concentrates of fibrinogen and factor VIII"],"prefix":"10.1111","volume":"86","author":[{"given":"P. R.","family":"Foster","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B. 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