{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,21]],"date-time":"2026-08-21T11:38:07Z","timestamp":1787312287086,"version":"build-2736575974"},"reference-count":46,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2004,6,15]],"date-time":"2004-06-15T00:00:00Z","timestamp":1087257600000},"content-version":"vor","delay-in-days":166,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Parasite Immunology"],"published-print":{"date-parts":[[2004,1]]},"abstract":"<jats:title>Summary<\/jats:title>\n                  <jats:p>\n                    <jats:italic>Vaccination of mice with a recombinant protein,<\/jats:italic>\n                    Ov\n                    <jats:italic>\u2010ASP\u20101, the<\/jats:italic>\n                    Onchocerca volvulus\n                    <jats:italic>homologue of the activation associated secreted gene family stimulated very high titres of both IgG1 and IgG2a without adjuvant. r<\/jats:italic>\n                    Ov\n                    <jats:italic>\u2010ASP\u20101 was also immuno\u2010reactive with IgG isotypes from both<\/jats:italic>\n                    O.\u00a0volvulus\n                    <jats:italic>\u2010infected (INF) and putatively immune (PI) humans, with higher IgG4 in the former group. The protein also stimulated IFN\u2010\u03b3 secretion by PBMC from INF and PI and IL\u20105 only in INF. Using a mouse diffusion chamber model, vaccination with r<\/jats:italic>\n                    Ov\n                    <jats:italic>\u2010ASP\u20101 resulted in partial but significant protection against challenge with infective third\u2010stage larvae (L3) but only when formulated with Freund's complete adjuvant (FCA) or alum. Protection was Th1\u2010dependent (highly elevated IgG2a) with FCA and contingent on a strongly Th2\u2010skewed (IgG1) response with alum. IgE responses to r<\/jats:italic>\n                    Ov\n                    <jats:italic>\u2010ASP\u20101 with or without adjuvant were weak or absent. When immunization using r<\/jats:italic>\n                    Ov\n                    <jats:italic>\u2010ASP\u20101 in adjuvant failed to induce adequate Th1 (FCA) or Th2 (alum) responses, protection efficacy was compromised. The recombinant protein appears to stimulate a mixed Th1\/Th2 response but the outcome in terms of protective immunity is the result of a subtle interplay of its intrinsic and adjuvant\u2010augmented properties.<\/jats:italic>\n                    Ov\n                    <jats:italic>\u2010ASP\u20101 is potentially secreted based on its localization in the secretory granules of L3.<\/jats:italic>\n                  <\/jats:p>","DOI":"10.1111\/j.0141-9838.2004.00685.x","type":"journal-article","created":{"date-parts":[[2004,6,15]],"date-time":"2004-06-15T15:42:00Z","timestamp":1087314120000},"page":"53-62","source":"Crossref","is-referenced-by-count":47,"title":["<i>Ov<\/i>\n                    \u2010ASP\u20101, the\n                    <i>Onchocerca volvulus<\/i>\n                    homologue of the activation associated secreted protein family is immunostimulatory and can induce protective anti\u2010larval immunity"],"prefix":"10.1111","volume":"26","author":[{"given":"A. 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