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A construct displays the same effects as the native material. It remains to be seen if MLIF is used, or even produced<\/jats:italic> in vivo <jats:italic>by the tissue\u2010invading parasite. If MLIF were to be relevant in invasive amoebiasis, immunizing against it could diminish this parasite advantage and prevent lesions. KLH\u2010linked MLIF mixed with Freund's adjuvant was too aggressive an immunizing material to answer this question. However, immunization with a tetramer of MLIF (but not a<\/jats:italic> scrambled <jats:italic>version of MLIF) around a lysine core (MLIF\u2013MAPS), that displays increased antigenicity, yet lacks excessive innate immunity activation, completely protects gerbils against amoebic abscess of the liver caused by the intraportal injection of virulent<\/jats:italic> E.\u00a0histolytica. <jats:italic>Liver abscesses caused by<\/jats:italic> Listeria monocytogenes <jats:italic>were not prevented. Invasive<\/jats:italic> E.\u00a0histolytica <jats:italic>may produce the parent protein of MLIF<\/jats:italic> in vivo<jats:italic>, and if appropriately cleaved, it may play a role in invasive amoebiasis. MLIF may join new vaccination strategies against amoebiasis.<\/jats:italic><\/jats:p>","DOI":"10.1111\/j.0141-9838.2004.00718.x","type":"journal-article","created":{"date-parts":[[2005,1,28]],"date-time":"2005-01-28T18:33:28Z","timestamp":1106937208000},"page":"343-349","source":"Crossref","is-referenced-by-count":11,"title":["Immunization with a tetramer derivative of an anti\u2010inflammatory pentapeptide produced by <i>Entamoeba histolytica<\/i> protects gerbils (<i>Meriones unguiculatus<\/i>) against experimental amoebic abscess of the liver"],"prefix":"10.1111","volume":"26","author":[{"given":"Juan Antonio","family":"Gim\u00e9nez\u2010Scherer","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guadalupe","family":"C\u00e1rdenas","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Martha","family":"L\u00f3pez\u2010Osuna","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Juan Raymundo","family":"Vel\u00e1zquez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guadalupe","family":"Rico","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Armando","family":"Isibasi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ma. del Carmen","family":"Maldonado","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ma. 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