{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T07:44:21Z","timestamp":1792741461510,"version":"4.5.0"},"reference-count":54,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2005,3,4]],"date-time":"2005-03-04T00:00:00Z","timestamp":1109894400000},"content-version":"vor","delay-in-days":3,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["resjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Medical Vet Entomology"],"published-print":{"date-parts":[[2005,3]]},"abstract":"<jats:p>\n                    <jats:bold>Abstract.\u2002<\/jats:bold>\n                    <jats:italic>Amblyomma americanum<\/jats:italic>\n                    (Linneaus) (Acari: Ixodidae), an important tick vector of human and animal disease, is not a competent vector of the bacterial agent of Lyme disease,\n                    <jats:italic>Borrelia burgdorferi<\/jats:italic>\n                    , although its range overlaps the geographical distribution of Lyme disease within the United States. A possible mechanism that could prevent acquisition of\n                    <jats:italic>B. burgdorferi<\/jats:italic>\n                    spirochetes from infected hosts is the toxic effect of\n                    <jats:italic>A. americanum<\/jats:italic>\n                    saliva on\n                    <jats:italic>B. burgdorferi<\/jats:italic>\n                    . The data presented here indicate that after 24 and 48\u2003h of exposure to\n                    <jats:italic>A. americanum<\/jats:italic>\n                    saliva, significantly fewer\n                    <jats:italic>B. burgdorferi<\/jats:italic>\n                    were alive compared to treatment controls as assessed by spirochete motility under dark\u2010field microscopy and resistance to the dead stain, propidium iodide. After 48\u2003h, fewer than 13% of saliva\u2010exposed\n                    <jats:italic>B. burgdorferi<\/jats:italic>\n                    were alive. In contrast, significantly more\n                    <jats:italic>B. burgdorferi<\/jats:italic>\n                    exposed to\n                    <jats:italic>Ixodes scapularis<\/jats:italic>\n                    (Acari: Ixodidae) saliva survived after 24 or 48\u2003h compared to\n                    <jats:italic>A. americanum<\/jats:italic>\n                    saliva or treatment controls.\n                  <\/jats:p>","DOI":"10.1111\/j.0269-283x.2005.00546.x","type":"journal-article","created":{"date-parts":[[2005,3,4]],"date-time":"2005-03-04T09:46:45Z","timestamp":1109929605000},"page":"90-95","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":26,"title":["Borreliacidal activity of saliva of the tick\n                    <i>Amblyomma americanum<\/i>"],"prefix":"10.1111","volume":"19","author":[{"given":"K. 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