{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T05:28:25Z","timestamp":1791091705109,"version":"4.1.0"},"reference-count":26,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2004,3,18]],"date-time":"2004-03-18T00:00:00Z","timestamp":1079568000000},"content-version":"vor","delay-in-days":17,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Scand J Immunol"],"published-print":{"date-parts":[[2004,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Innate mechanisms involving natural killer cells have been implied to play an important role in immunity against <jats:italic>Leishmania<\/jats:italic> infection. Previous studies have evaluated responses to three purified amastigote antigens, P\u20102, P\u20104 and P\u20108, of <jats:italic>Leishmania pifanoi<\/jats:italic>. The P\u20104 and P\u20108 antigens have been demonstrated to induce protection in mouse models, as well as to induce cellular responses in American cutaneous leishmaniasis patients. Cells from <jats:italic>Leishmania aethiopica<\/jats:italic>\u2010infected leishmaniasis patients preferentially responded to P\u20108 and, to a lesser extent, to the cysteine proteinase, P\u20102. In this study, it is shown that cells from healthy donors, including cells from truly na\u00efve donors (cord blood), could be stimulated to proliferation and cytokine production by P\u20102. The main proliferating cell types in healthy adult donors were CD16\/56<jats:sup>+<\/jats:sup> and the CD8<jats:sup>+<\/jats:sup> cells. Blocking of major histocompatibility complex (MHC) class II with \u03b1\u2010MHC class II antibodies markedly inhibited proliferation and interferon\u2010\u03b3 (IFN\u2010\u03b3) production, whereas interleukin\u201010 production was not affected. Experimental evidence indicates that CD4<jats:sup>+<\/jats:sup> cells were not necessary for the proliferative and IFN\u2010\u03b3 responses; however, an adherent cell population was required. Furthermore, CD16\/56<jats:sup>+<\/jats:sup> cells expressing MHC class II were expanded following P\u20102 stimulation. The responses to P\u20102 show a striking similarity to responses induced by the vaccine candidate <jats:italic>Leishmania<\/jats:italic> homologue of receptors for activated C\u2010kinase (LACK) in healthy donors. The responses described here may not be desirable when aiming at inducing protective immune responses with a vaccine, and the implications of these results for the development of vaccines against leishmaniasis are discussed.<\/jats:p>","DOI":"10.1111\/j.0300-9475.2004.01388.x","type":"journal-article","created":{"date-parts":[[2004,3,18]],"date-time":"2004-03-18T14:18:02Z","timestamp":1079619482000},"page":"294-304","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Leishmanial Amastigote Antigen P\u20102 Induces Major Histocompatibility Complex Class II\u2010Dependent Natural Killer\u2010Cell Reactivity in Cells from Healthy Donors"],"prefix":"10.1111","volume":"59","author":[{"given":"S.","family":"Nyl\u00e9n","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K.","family":"Maasho","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D.","family":"McMahon\u2010Pratt","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H.","family":"Akuffo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2004,3,18]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.178.2.567"},{"key":"e_1_2_6_3_2","doi-asserted-by":"crossref","first-page":"2698","DOI":"10.1128\/IAI.66.6.2698-2704.1998","article-title":"Indication of the protective role of natural killer cells in human cutaneous leishmaniasis in an area of endemicity","volume":"66","author":"Maasho K","year":"1998","journal-title":"Infect Immun"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2249.1993.tb08227.x"},{"key":"e_1_2_6_5_2","doi-asserted-by":"crossref","first-page":"2406","DOI":"10.4049\/jimmunol.140.7.2406","article-title":"Monoclonal antibodies specific for the amastigote stage of Leishmania pifanoi. 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