{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T12:27:21Z","timestamp":1791116841221,"version":"4.1.0"},"reference-count":63,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2004,10,15]],"date-time":"2004-10-15T00:00:00Z","timestamp":1097798400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Cutan Pathol"],"published-print":{"date-parts":[[2004,11]]},"abstract":"<jats:p><jats:bold>Background:\u2002<\/jats:bold> The hyperproliferative keratinocytic lesions encompass a wide range of non\u2010tumorigenic, pretumorigenic, and tumorigenic conditions. The aim of this work was to examine the expression patterns of apoptosis\u2010linked molecules (bcl\u20102 and p53) in these lesions.<\/jats:p><jats:p><jats:bold>Methods:\u2002<\/jats:bold> Immunoperoxidase staining methods were applied to analyze p53 and bcl\u20102 protein expression in a total of 66 cases, including 12 squamous cell carcinomas (both <jats:italic>in situ<\/jats:italic> and invasive SCC), 11 actinic keratoses (AK), 13 psoriasis vulgaris (PV), eight verruca vulgaris (VV), six chronic dermatitis (CD), five seborrheic keratosis (SK), four lichen planus (LP), three epidermodysplasia verruciformis (EDV), two condyloma acuminata (CA), two lichen simplex chronicus (LSC), and 10 specimens from normal skin.<\/jats:p><jats:p><jats:bold>Results:\u2002<\/jats:bold> As compared to normal skin (0.70\u2003\u00b1\u20030.26), the bcl\u20102 average weighted scores in the non\u2010tumorigenic (0.76\u2003\u00b1\u20030.16), pretumorigenic (1.45\u2003\u00b1\u20030.28), and tumorigenic lesions (2.83\u2003\u00b1\u20030.50 and 2.92\u2003\u00b1\u20030.50 for <jats:italic>in situ<\/jats:italic> and invasive SCC, respectively) showed significant up\u2010regulation (p\u2003=\u20030.001). In the non\u2010tumorigenic lesions, the bcl\u20102 expression values decreased in the following order: SK\u2003&gt;\u2003EDV\u2003&gt;\u2003CD\u2003&gt;\u2003LP\u2003&gt;\u2003CA\u2003&gt; PV\u2003&gt;\u2003VV (1.40\u2003\u00b1\u20030.24\u2003&gt;\u20031.33\u2003\u00b1\u20030.67\u2003&gt;\u20030.83\u2003\u00b1\u20030.40\u2003&gt;\u20030.67\u2003\u00b1\u20030.21\u2003&gt; 0.50\u2003\u00b1\u20030.20\u2003&gt;\u20030.46\u2003\u00b1\u20030.22\u2003&gt;\u20030.13\u2003\u00b1\u20030.01, respectively). As compared to normal skin (1.10\u2003\u00b1\u20030.23), the p53 average weighted scores in the non\u2010tumorigenic (1.86\u2003\u00b1\u20030.18), pretumorigenic (3.64\u2003\u00b1\u20030.53), and tumorigenic lesions (5.00\u2003\u00b1\u20031.00 and 5.08\u2003\u00b1\u20030.86 for <jats:italic>in situ<\/jats:italic> and invasive SCC, respectively) showed significant up\u2010regulation (p\u2003=\u20030.021). In the non\u2010tumorigenic lesions, p53 average weighted scores decreased in the following order: SK\u2003&gt;\u2003PV\u2003&gt;\u2003CA\u2003&gt;\u2003LP\u2003&gt;\u2003CD\u2003&gt; VV\u2003&gt;\u2003EDV (3.20\u2003\u00b1\u20030.49\u2003&gt;\u20032.38\u2003\u00b1\u20030.27\u2003&gt;\u20032.0\u2003\u00b1\u20030.0\u2003&gt;\u20031.83\u2003\u00b1\u20030.48\u2003&gt; 1.0\u2003\u00b1\u20030.37\u2003&gt;\u20031.0\u2003\u00b1\u20030.33\u2003&gt;\u20031.0\u2003\u00b1\u20030.0, respectively). There was a positive correlation between bcl\u20102 and p53 protein expression in normal skin (r\u2003=\u20030.966, p\u2003=\u20030.0001), non\u2010tumorigenic (r\u2003=\u20030.775, p\u2003=\u20030.0001), pretumorigenic (r\u2003=\u20030.830, p\u2003=\u20030.001), and tumorigenic lesions (r\u2003=\u20030.757, p\u2003=\u20030.003).<\/jats:p><jats:p><jats:bold>Conclusions:\u2002<\/jats:bold> Bcl\u20102 and p53 proteins are altered in the keratinocytic hyperproliferative lesions. Determination of whether these alterations reflect underlying gene mutations will require further investigations.<\/jats:p>","DOI":"10.1111\/j.0303-6987.2004.00244.x","type":"journal-article","created":{"date-parts":[[2004,10,15]],"date-time":"2004-10-15T10:19:54Z","timestamp":1097835594000},"page":"643-651","source":"Crossref","is-referenced-by-count":53,"title":["Analysis of p53 and bcl\u20102 protein expression in the non\u2010tumorigenic, pretumorigenic, and tumorigenic keratinocytic hyperproliferative lesions"],"prefix":"10.1111","volume":"31","author":[{"given":"Mahmoud R.","family":"Hussein","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zaeneb H.","family":"Al\u2010Badaiwy","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcelle 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