{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,26]],"date-time":"2026-03-26T04:27:45Z","timestamp":1774499265749,"version":"3.50.1"},"reference-count":40,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2004,12,15]],"date-time":"2004-12-15T00:00:00Z","timestamp":1103068800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Cutan Pathol"],"published-print":{"date-parts":[[2005,1]]},"abstract":"<jats:p>The human \u03b3\u2010herpes virus\u20108 (HHV\u20108) was first described in AIDS\u2010related Kaposi's sarcoma (KS) tumour samples. In this study, we report comparative studies on paraffin\u2010embedded biopsies of AIDS\u2010related KS (AKS) and endemic KS (EKS) with regard to HHV\u20108 content as evaluated using polymerase chain reaction (PCR) and immunohistochemistry. DNA was extracted either using Chelex\u2010100 or using Qia\u2010gene kit and was evaluated with the help of a semiquantitative PCR assay. The PCR detection of HHV\u20108 was more sensitive to the Chelex method than to Qia\u2010gene. The threshold for PCR test sensitivity with the help of serial dilution of DNA was at the level of five plasmid ORF\u201026 regions, and DNA from 25 body cavity\u2010based lymphoma\u20101 cells. The results expressed as virus load\/actin unit showed progressively higher HHV\u20108 levels in late (nodular) cases, compared to those in early (patch\/plaque) stages. Evaluation of HHV\u20108 DNA levels in tumour tissues, thus, indicates a correlation between virus load and KS stage. Double immunostaining of spindle cells (SC) in KS biopsies for CD34 and HHV\u20108\/latency\u2010associated nuclear antigen (LANA) showed an increase in double\u2010positive SC in the lesions of nodular AKS and EKS cases, compared to that in plaque and patch stages. However, 10\u201315% of CD34<jats:sup>+<\/jats:sup>\/LANA<jats:sup>\u2013<\/jats:sup> SC cells were observed during the development from patch to nodular cases of AKS and EKS. Our results indicate that PCR analysis is a simple and sensitive diagnostic method for HHV\u20108 evaluation in KS tissues, processed for conventional histopathology.<\/jats:p>","DOI":"10.1111\/j.0303-6987.2005.00256.x","type":"journal-article","created":{"date-parts":[[2004,12,15]],"date-time":"2004-12-15T14:27:05Z","timestamp":1103120825000},"page":"21-27","source":"Crossref","is-referenced-by-count":32,"title":["HHV\u20108\/KSHV during the development of Kaposi's sarcoma: evaluation by polymerase chain reaction and immunohistochemistry"],"prefix":"10.1111","volume":"32","author":[{"given":"Fatemeh","family":"Pak","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pawan","family":"Pyakural","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Parviz","family":"Kokhaei","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ephata","family":"Kaaya","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A. 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