{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,2]],"date-time":"2026-07-02T01:01:55Z","timestamp":1782954115483,"version":"3.54.5"},"reference-count":18,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2004,2,9]],"date-time":"2004-02-09T00:00:00Z","timestamp":1076284800000},"content-version":"vor","delay-in-days":8,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Experimental Dermatology"],"published-print":{"date-parts":[[2004,2]]},"abstract":"<jats:p><jats:bold>Abstract:<\/jats:bold>  <jats:italic>APAF\u20101<\/jats:italic> plays a pivotal role in mitochondria\u2010dependent apoptosis, binding to cytochrome c and favoring activation of caspase\u20109. It has been shown that epigenetic silencing of the <jats:italic>APAF\u20101<\/jats:italic> gene is a common event in several metastatic melanoma cells <jats:italic>in vitro<\/jats:italic>. We determined, by Western blot, variation in the level of expression of <jats:italic>APAF\u20101<\/jats:italic> in several human melanoma cell lines and, by immunohistochemistry, in a group of 106 histological samples including benign and malignant melanocytic lesions. We observed <jats:italic>APAF\u20101<\/jats:italic> down\u2010regulation or loss of expression in two metastatic melanoma cell lines, compared to primary melanoma cell lines. The immunohistochemical analysis revealed a significant difference in <jats:italic>APAF\u20101<\/jats:italic> staining between nevi and melanomas. In addition, we found a significant negative correlation between <jats:italic>APAF\u20101<\/jats:italic> expression level and tumor thickness and between primary melanomas and metastases. We conclude that loss of <jats:italic>APAF\u20101<\/jats:italic> expression can be considered as an indicator of malignant transformation in melanoma.<\/jats:p>","DOI":"10.1111\/j.0906-6705.2004.00136.x","type":"journal-article","created":{"date-parts":[[2004,2,9]],"date-time":"2004-02-09T08:49:01Z","timestamp":1076316541000},"page":"93-97","source":"Crossref","is-referenced-by-count":50,"title":["Analysis of <i>APAF\u20101<\/i> expression in human cutaneous melanoma 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