{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,19]],"date-time":"2026-09-19T19:45:57Z","timestamp":1789847157834,"version":"4.0.1"},"reference-count":16,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2004,6,25]],"date-time":"2004-06-25T00:00:00Z","timestamp":1088121600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Experimental Dermatology"],"published-print":{"date-parts":[[2004,7]]},"abstract":"<jats:p><jats:bold>Abstract:\u2002<\/jats:bold> Mycosis fungoides (MF) is the most frequent variant of cutaneous T\u2010cell lymphomas (CTCLs). MF primarily involves the skin initially with patches and plaques. In later stages, cutaneous tumors develop and tumor cells may spread to lymph nodes and finally to visceral sites. Here, we describe an animal model for MF in immune\u2010deficient nude mice, using the CTCL cell line MyLa. Subcutaneous transplantation of MyLa cells leads to the formation of cutaneous tumors in 80% of the mice (50\/60 total). Spread of tumor cells to visceral sites was detected by immunohistochemistry and polymerase chain reaction (PCR)\u2010based detection of specific T\u2010cell receptor\u2010\u03b3 rearrangement. MyLa cells were found circulating in the blood, lymph nodes, and in blood vessels of heart, kidney, lung, and liver. In lung and liver tissue, tumor cells presented perivascular invasion, but no large secondary tumors developed. The nude mouse model described here will be a valuable test system for new therapeutic approaches for the treatment of MF and opens the unique opportunity to study the disease <jats:italic>in vivo<\/jats:italic>.<\/jats:p>","DOI":"10.1111\/j.0906-6705.2004.00201.x","type":"journal-article","created":{"date-parts":[[2004,6,25]],"date-time":"2004-06-25T08:04:05Z","timestamp":1088150645000},"page":"406-412","source":"Crossref","is-referenced-by-count":29,"title":["Establishment of a mouse xenograft model for mycosis fungoides"],"prefix":"10.1111","volume":"13","author":[{"given":"Sonja","family":"Thaler","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Angelika M.","family":"Burger","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas","family":"Schulz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Boris","family":"Brill","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexandra","family":"Bittner","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrick A.","family":"Oberholzer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Reinhard","family":"Dummer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Barbara S.","family":"Schnierle","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2004,6,25]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1053\/sder.2000.8339"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0889-8588(18)30053-4"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1111\/1523-1747.ep12280303"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V88.7.2385.bloodjournal8872385"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00431220"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF02631086"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1111\/1523-1747.ep12874500"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0733-8635(18)30176-1"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V92.1.252.413k08_252_258"},{"key":"e_1_2_6_11_2","volume-title":"Current Protocols in Immunology","author":"Coligan J E","year":"1999"},{"key":"e_1_2_6_12_2","volume-title":"Current Protocols in Immunology","author":"Ausubel F M","year":"1988"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.87.21.8536"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0625.1997.tb00158.x"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1993.tb05798.x"},{"key":"e_1_2_6_16_2","first-page":"61","article-title":"Mycosis fungoides","volume":"51","author":"Epstein E H","year":"1972","journal-title":"Survival, prognostic features, response to therapy, and autopsy findings"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.7326\/0003-4819-93-2-223"}],"container-title":["Experimental Dermatology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.0906-6705.2004.00201.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.0906-6705.2004.00201.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,26]],"date-time":"2023-09-26T22:29:11Z","timestamp":1695767351000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.0906-6705.2004.00201.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2004,6,25]]},"references-count":16,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2004,7]]}},"alternative-id":["10.1111\/j.0906-6705.2004.00201.x"],"URL":"https:\/\/doi.org\/10.1111\/j.0906-6705.2004.00201.x","archive":["Portico"],"relation":{},"ISSN":["0906-6705","1600-0625"],"issn-type":[{"value":"0906-6705","type":"print"},{"value":"1600-0625","type":"electronic"}],"subject":[],"published":{"date-parts":[[2004,6,25]]}}}