{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T21:08:59Z","timestamp":1760908139392},"reference-count":18,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2004,9,24]],"date-time":"2004-09-24T00:00:00Z","timestamp":1095984000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Experimental Dermatology"],"published-print":{"date-parts":[[2004,10]]},"abstract":"<jats:p><jats:bold>Abstract:\u2002<\/jats:bold> The aim of this work was to study the effects and side effects of gemcitabine (2\u2032,2\u2032\u2010difluorodeoxycytidine, dFdC), a pyrimidine synthesis inhibitor, on skin lesions of a herpes simplex virus (HSV)\u2010induced Adamantiades\u2013Beh\u00e7et's disease (ABD)\u2010like mouse model. For the dose\u2010escalation study, ICR mice were treated intraperitoneally with dFdC over 5 days. For the efficacy study, ICR mice were inoculated with HSV and classified as having ABD according to a revised Japanese classification, and then 18 ABD mice were randomly assigned to placebo, 0.06 or 0.12\u2003\u00b5g of dFdC\/day over 5 days. Serum levels of interleukin\u20104 (IL\u20104), IL\u20106, IL\u201010, interferon\u2010\u03b3 (IFN\u2010\u03b3), and tumor necrosis factor\u2010\u03b1 (TNF\u2010\u03b1) were determined using enzyme\u2010linked immunosorbent assay. After application of 3\u2003\u00b5g of dFdC over 5 days, alanine aminotransferase increased (<jats:italic>P<\/jats:italic>\u2003=\u20030.032), but all other kidney and liver parameters were unchanged. In ABD mice, 5 days of dFdC treatment with 0.06 or 0.12\u2003\u00b5g of dFdC\/day resulted in a dose\u2010dependent improvement of cutaneous manifestations by more than 60% (<jats:italic>P<\/jats:italic>\u2003=\u20030.017). There was no significant change in cytokine levels, and none of the cytokine levels correlated with response to treatment. Moreover, dFdC shows promising effects to improve cutaneous lesions in the HSV\u2010induced ABD\u2010like mouse model. In this animal model, effects of dFdC on the cytokine profile remained inconclusive.<\/jats:p>","DOI":"10.1111\/j.0906-6705.2004.00210.x","type":"journal-article","created":{"date-parts":[[2004,9,24]],"date-time":"2004-09-24T13:18:10Z","timestamp":1096031890000},"page":"630-634","source":"Crossref","is-referenced-by-count":7,"title":["Therapeutic effects of gemcitabine on cutaneous manifestations in an Adamantiades\u2013Beh\u00e7et's disease\u2010like mouse model"],"prefix":"10.1111","volume":"13","author":[{"given":"Seonghyang","family":"Sohn","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Manfred","family":"Lutz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hyuk Jae","family":"Kwon","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guenther","family":"Konwalinka","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sungnack","family":"Lee","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael","family":"Schirmer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2004,9,24]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199910213411707"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0190-9622(99)80049-8"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00430-002-0167-5"},{"key":"e_1_2_6_5_2","first-page":"21","article-title":"Beh\u00e7et's disease\u2010like symptoms induced by the herpes simplex virus in ICR mice","volume":"8","author":"Sohn S","year":"1998","journal-title":"Eur J Dermatol"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0923-1811(01)00086-X"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2133.2001.04498.x"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1086\/319681"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1097\/00001813-199512006-00002"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1038\/bjc.1998.747"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1097\/00001622-200111000-00015"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0145-2126(99)00142-3"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1097\/00007890-199910150-00025"},{"key":"e_1_2_6_14_2","first-page":"321","article-title":"Cytokines in Beh\u00e7et's disease","volume":"23","author":"Sayinalp N","year":"1996","journal-title":"J Rheumatol"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1159\/000068888"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/0923-1811(95)00439-4"},{"key":"e_1_2_6_17_2","first-page":"321","article-title":"Cytokines in Beh\u00e7et's disease","volume":"23","author":"Sayinalp N","year":"1996","journal-title":"J Rheumatol"},{"key":"e_1_2_6_18_2","doi-asserted-by":"publisher","DOI":"10.1002\/1529-0131(199909)42:9<1967::AID-ANR24>3.0.CO;2-Z"},{"key":"e_1_2_6_19_2","first-page":"329","article-title":"Divergent cytokine production profile in Beh\u00e7et's disease. Altered Th1\/Th2 cell cytokine pattern","volume":"25","author":"Raziuddin S","year":"1998","journal-title":"J Rheumatol"}],"container-title":["Experimental Dermatology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.0906-6705.2004.00210.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.0906-6705.2004.00210.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,11]],"date-time":"2023-10-11T01:43:38Z","timestamp":1696988618000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.0906-6705.2004.00210.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2004,9,24]]},"references-count":18,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2004,10]]}},"alternative-id":["10.1111\/j.0906-6705.2004.00210.x"],"URL":"https:\/\/doi.org\/10.1111\/j.0906-6705.2004.00210.x","archive":["Portico"],"relation":{},"ISSN":["0906-6705","1600-0625"],"issn-type":[{"value":"0906-6705","type":"print"},{"value":"1600-0625","type":"electronic"}],"subject":[],"published":{"date-parts":[[2004,9,24]]}}}