{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T00:04:14Z","timestamp":1761609854715},"reference-count":24,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2004,11,18]],"date-time":"2004-11-18T00:00:00Z","timestamp":1100736000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Experimental Dermatology"],"published-print":{"date-parts":[[2004,12]]},"abstract":"<jats:p><jats:bold>Abstract:\u2002<\/jats:bold> Interferon regulatory factors (IRFs) are a family of transcriptional factors induced by interferon\u2010\u03b3 (IFN\u2010\u03b3). Recent studies have indicated that the deregulation of IRF system in keratinocytes is responsible, at least in part, for aberrant proliferation and the differentiation of the psoriatic epidermis. Previously, we reported that the expression of 230\u2010kDa bullous pemphigoid antigen (<jats:italic>BPAG1<\/jats:italic>) gene, which is strictly restricted to basal keratinocytes, is transcriptionally suppressed by IFN\u2010\u03b3, but the contribution of IRFs in such suppression is still unclear. In this study, we investigated the role of IRFs in the regulation of BPAG1 gene expression. Computer analysis identified IRF1 and IRF2 consensus sequences between \u2212135 and \u2212123 on BPAG1 promoter region. Transient transfection studies with BPAG1 promoter\u2010luciferase reporter gene plasmids and IRF1 and IRF2 expression plasmids revealed that IRF1 and IRF2 directly down\u2010regulated BPAG1 gene transcription in cultured normal human epidermal keratinocytes. Several sets of gel retardation assays with the BPAG1\u2013IRF binding sequence as a probe indicated that IRF1 and IRF2 could bind to the BPAG1\u2013IRF sequence, but some other protein(s), which was induced by IFN\u2010\u03b3 stimulation and possessed binding activity to IRF consensus sequence, showed preferential binding to the BPAG1\u2013IRF sequence. Our results suggest that IFN\u2010\u03b3\u2013IRF system is involved in BPAG1 gene regulation in type\u20101 helper T\u2010cell inflammatory skin conditions, such as psoriasis vulgaris.<\/jats:p>","DOI":"10.1111\/j.0906-6705.2004.00219.x","type":"journal-article","created":{"date-parts":[[2004,11,18]],"date-time":"2004-11-18T13:34:54Z","timestamp":1100784894000},"page":"773-779","source":"Crossref","is-referenced-by-count":9,"title":["Transcriptional regulation of the 230\u2010kDa bullous pemphigoid antigen gene expression by interferon regulatory factor 1 and interferon regulatory factor 2 in normal human epidermal keratinocytes"],"prefix":"10.1111","volume":"13","author":[{"given":"Maiko","family":"Odanagi","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yasushi","family":"Kikuchi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takehiko","family":"Yamazaki","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takahide","family":"Kaneko","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hajime","family":"Nakano","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Katsuto","family":"Tamai","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joani","family":"Vitto","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Katsumi","family":"Hanada","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2004,11,18]]},"reference":[{"key":"e_1_2_5_2_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.immunol.15.1.749"},{"key":"e_1_2_5_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)42719-6"},{"key":"e_1_2_5_4_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.167.4.1323"},{"key":"e_1_2_5_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0190-9622(89)70073-6"},{"key":"e_1_2_5_6_2","doi-asserted-by":"crossref","first-page":"2016","DOI":"10.1016\/S0021-9258(17)42129-6","article-title":"Control of growth regulatory and differentiation\u2010specific genes in human epidermal keratinocytes by interferon gamma. 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