{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,24]],"date-time":"2026-10-24T15:31:20Z","timestamp":1792855880878,"version":"4.5.0"},"reference-count":49,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2004,11,18]],"date-time":"2004-11-18T00:00:00Z","timestamp":1100736000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Experimental Dermatology"],"published-print":{"date-parts":[[2004,12]]},"abstract":"<jats:p><jats:bold>Abstract:\u2002<\/jats:bold> Narrow\u2010band ultraviolet\u2010B (UVB) radiation is an effective treatment for vitiligo vulgaris. However, the mechanisms of narrow\u2010band UVB in inducing repigmentation of vitiligo lesions are not thoroughly clarified. The purpose of our study was to investigate the effects of narrow\u2010band UVB irradiation on melanocyte proliferation and migration <jats:italic>in vitro<\/jats:italic>. Our results showed that the cell counts as well as [3H]thymidine uptake of melanocytes were significantly enhanced by narrow\u2010band UVB\u2010irradiated keratinocyte supernatants. In these supernatants, a significant increase in basic fibroblast growth factor (bFGF) and in endothelin\u20101 (ET\u20101) release was observed. bFGF is a natural mitogen for melanocytes, whereas ET\u20101 can stimulate DNA synthesis in melanocytes. This stimulatory effect of melanocyte proliferation by supernatants derived from narrow\u2010band UVB\u2010irradiated keratinocytes was significantly reduced by a selective endothelin\u2010B (ET\u2010B) receptor antagonist (BQ788), suggesting an essential role of ET\u20101 on melanocyte proliferation. Our results of time\u2010lapse microphotography revealed a stimulatory effect of narrow\u2010band UVB irradiation on melanocyte migration. Focal adhesion kinase (FAK) plays a pivotal role in cell migration. Phosphorylated FAK (p125<jats:sup>FAK<\/jats:sup>) expression on melanocyte was enhanced by narrow\u2010band UVB irradiation. In this study, narrow\u2010band UVB irradiation stimulated a significant increase in matrix metalloproteinase\u20102 (MMP\u20102) activity in melanocyte supernatants. Narrow\u2010band UVB\u2010irradiation\u2010induced migration of melanocytes was significantly annihilated by the addition of p125<jats:sup>FAK<\/jats:sup> inhibitor (herbimycin\u2010A) or MMP\u20102 inhibitor (GM6001). These results suggest that p125<jats:sup>FAK<\/jats:sup> and MMP\u20102 activity play important roles in narrow\u2010band UVB\u2010induced migration of melanocytes. Our results provide a theoretical basis for the effectiveness of narrow\u2010band UVB irradiation in treating vitiligo.<\/jats:p>","DOI":"10.1111\/j.0906-6705.2004.00221.x","type":"journal-article","created":{"date-parts":[[2004,11,18]],"date-time":"2004-11-18T13:34:54Z","timestamp":1100784894000},"page":"755-763","source":"Crossref","is-referenced-by-count":104,"title":["Narrow\u2010band ultraviolet\u2010B stimulates proliferation and migration of cultured melanocytes"],"prefix":"10.1111","volume":"13","author":[{"given":"Ching\u2010Shuang","family":"Wu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chia\u2010Li","family":"Yu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chieh\u2010Shan","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cheng\u2010Che 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