{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T01:07:06Z","timestamp":1791248826365,"version":"4.1.0"},"reference-count":36,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2005,4,25]],"date-time":"2005-04-25T00:00:00Z","timestamp":1114387200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Experimental Dermatology"],"published-print":{"date-parts":[[2005,5]]},"abstract":"<jats:p><jats:bold>Abstract:\u2002<\/jats:bold> We, in this study, describe how T lymphocytes in a skin biopsy can proliferate <jats:italic>in vitro<\/jats:italic> for up to 3\u2003months by using T\u2010cell growth factors \u2013 interleukin\u20102 (IL\u20102) and IL\u20104 yielding approximately 100\u2013160 million T lymphocytes within 1\u2003month. We established cell lines from three tuberculin skin tests, four positive patch tests, 15 of 16 biopsies from atopic dermatitis (AD), 15 of 19 biopsies from mycosis fungoides (MF), 12 of 24 biopsies from psoriasis vulgaris, which was significantly less than AD (<jats:italic>P\u2003&lt;<\/jats:italic>\u20030.05), and with a reduced cumulative number of lymphocytes (<jats:italic>P\u2003&lt;<\/jats:italic>\u20030.05). Omitting IL\u20102 and IL\u20104 led to immediate halt of proliferation. Blood mononuclear cells from patients and biopsies from healthy persons never gave cell lines. All cells were T lymphocytes expressing CD45RO<jats:sup>+<\/jats:sup>, HLA\u2010DR<jats:sup>+<\/jats:sup> and CD150. The CD7 expression was significantly increased in cell lines from AD (<jats:italic>P\u2003&lt;<\/jats:italic>\u20030.05). T\u2010cell receptor \u03b2\u2010chain studies by using reverse transcription\u2010polymerase chain reaction showed that all T lymphocytes had access to the skin compartment. Single\u2010stranded conformational analysis showed clonally expanded T cells numbering between 40 and 60 clones. After approximately 2\u2003months of growth, the mean CD4<jats:sup>+<\/jats:sup>\u2003:\u2003CD8<jats:sup>+<\/jats:sup> ratio was for AD 1.20, MF 0.65 and psoriasis 0.85. Patients with AD treated with cyclosporin\u2010A had almost no growth of CD8<jats:sup>+<\/jats:sup> cells <jats:italic>in vitro<\/jats:italic>. Our findings indicate a changed homeostasis among skin\u2010homing lymphocytes for <jats:italic>in vitro<\/jats:italic> culture. Our culture system of skin\u2010homing T lymphocytes leads to a prominent cellular expansion allowing for a range of studies of <jats:italic>in vivo<\/jats:italic> activated skin T lymphocytes.<\/jats:p>","DOI":"10.1111\/j.0906-6705.2005.00294.x","type":"journal-article","created":{"date-parts":[[2005,4,25]],"date-time":"2005-04-25T12:57:49Z","timestamp":1114433869000},"page":"391-397","source":"Crossref","is-referenced-by-count":10,"title":["<i>In vitro<\/i> culture of skin\u2010homing T lymphocytes from inflammatory skin diseases"],"prefix":"10.1111","volume":"14","author":[{"given":"Karen","family":"Bang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marianne","family":"Lund","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S\u00f8ren C.","family":"Mogensen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kristian","family":"Thestrup\u2010Pedersen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2005,4,25]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/0091-6749(83)90546-8"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2249.2000.01333.x"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.2340\/00015555924447"},{"key":"e_1_2_6_5_2","first-page":"160","article-title":"Aspects of the treatment of mycosis fungoides. 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