{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,7]],"date-time":"2025-11-07T13:09:52Z","timestamp":1762520992240},"reference-count":47,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2005,5,10]],"date-time":"2005-05-10T00:00:00Z","timestamp":1115683200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Experimental Dermatology"],"published-print":{"date-parts":[[2005,6]]},"abstract":"<jats:p><jats:bold>Abstract:\u2002<\/jats:bold> Nitric oxide (NO) is a reactive endogenous molecule with multiple functions including inflammation and immunity. NO stimulates melanogenesis by activating soluble guanylyl cyclase (sGC) resulting in increases in intracellular guanosine 3\u2032,5\u2032\u2010cyclic monophosphate (cGMP). <jats:italic>In vitro<\/jats:italic> experiments showed that NO could inhibit the <jats:italic>de novo<\/jats:italic> attachment of melanocytes to extracellular matrix (ECM) suggesting that NO\u2010induced aberrant perturbation of melanocyte\u2013ECM interaction could be a reason for melanocyte loss in vitiliginous lesions. Here, we examined whether there might be differences between normal melanocytes and vitiliginous melanocytes (VMs) with respect to NO\u2010induced detachment from ECM and whether cGMP is involved. We used the direct NO donor (<jats:italic>Z<\/jats:italic>)\u20101\u2010[<jats:italic>N<\/jats:italic>\u2010(3\u2010ammoniopropyl)\u2010<jats:italic>N<\/jats:italic>\u2010(<jats:italic>n<\/jats:italic>\u2010propyl)amino]diazen\u20101\u2010ium\u20101,2\u2010diolate and the peroxynitrite donor 3\u2010morpholino\u2010sydnonimine for the present studies. These donors induced detachment of both normal melanocytes and non\u2010lesional VMs in a time\u2010 and concentration\u2010dependent manner with comparable susceptibility and similar expression profile of sGC. Treatment of melanocytes with caspase inhibitors reduced cell detachment, indicating that a major part of the detachment is due to apoptosis. The NO\u2010induced detachment but not apoptosis was partly inhibited in the presence of sGC and cGMP\u2010dependent protein kinase inhibitors. In addition, the membrane\u2010permeable cGMP analog 8\u2010(4\u2010chlorophenyethio\/guanosine\u20103\u2032,5\u2032\u2010cyclic monophosphate (PCPT) cGMP was not able to induce apoptosis in melanocytes, suggesting that NO\u2010induced detachment of melanocytes via apoptosis is cGMP\u2010independent. The present results also indicate that there are no apparent differences between NO\u2010induced detachment of non\u2010lesional vitiliginous and normal melanocytes from ECM.<\/jats:p>","DOI":"10.1111\/j.0906-6705.2005.00302.x","type":"journal-article","created":{"date-parts":[[2005,5,10]],"date-time":"2005-05-10T08:06:10Z","timestamp":1115712370000},"page":"445-453","source":"Crossref","is-referenced-by-count":14,"title":["Non\u2010lesional vitiliginous melanocytes are not characterized by an increased proneness to nitric oxide\u2010induced apoptosis"],"prefix":"10.1111","volume":"14","author":[{"given":"Krassimira","family":"Ivanova","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rene","family":"Van Den 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