{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,6]],"date-time":"2025-11-06T19:47:14Z","timestamp":1762458434729},"reference-count":39,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2005,6,8]],"date-time":"2005-06-08T00:00:00Z","timestamp":1118188800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Experimental Dermatology"],"published-print":{"date-parts":[[2005,7]]},"abstract":"<jats:p><jats:bold>Abstract:\u2002<\/jats:bold> Using non\u2010self discrimination as a driving force in generating peptide immunogenicity, we have developed a computer\u2010assisted proteomic analysis in order to identify the protein antigenic regions that have evoked humoral response. The purpose of this study was to further validate the computational analysis for melanoma\u2010associated antigens and, at the same time, to assess the efficacy of the methodology in defining antigenic regions of autoantigens associated to autoimmune diseases. To achieve this two\u2010fold objective, we have examined the enzyme tyrosinase, a protein that represents an important autoantigen in patients with vitiligo or melanoma. Here, we report that the antigenic linear determinant of the monoclonal antibody (Mab) T311 raised against the melanoma\/vitiligo tyrosinase autoantigen is located in the low similarity 15\u2010mer amino acid sequence tyrosinase 233\u2013247 IPYWDWRDAEKCDIC<jats:italic>,<\/jats:italic> within the fragment 237\u2013247. These data confirm non\u2010similarity to the host proteome as a factor that participates in shaping peptide immune reactivity and may be a first step towards designing tyrosinase antigenic peptides to be used for (i) direct neutralization of harmful melanocytes\u2010attacking autoantibodies in vitiligo, or (ii) production of antibodies against tyrosinase\u2010positive melanomas. Moreover tyrosinase peptide antigens might be used as key tools in studying the boundaries between self\u2010tolerance and autoimmunity phenomena.<\/jats:p>","DOI":"10.1111\/j.0906-6705.2005.00327.x","type":"journal-article","created":{"date-parts":[[2005,6,8]],"date-time":"2005-06-08T08:16:30Z","timestamp":1118218590000},"page":"543-550","source":"Crossref","is-referenced-by-count":15,"title":["Definition of anti\u2010tyrosinase MAb T311 linear determinant by proteome\u2010based similarity analysis"],"prefix":"10.1111","volume":"14","author":[{"given":"J\u00f6rg","family":"Willers","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alberta","family":"Lucchese","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Abraham","family":"Mittelman","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Reinhard","family":"Dummer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Darja","family":"Kanduc","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,6,8]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1517\/14712598.4.2.181"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1568-9972(03)00061-2"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.2174\/1568010023344562"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1021\/ja039583d"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1021\/ja038058b"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.10186"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0196-9781(99)00095-9"},{"key":"e_1_2_6_9_2","first-page":"465","article-title":"Peptide mimotopes of oncoproteins as therapeutic agents in breast cancer","volume":"11","author":"Ashok B T","year":"2003","journal-title":"Int J Mol Med"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1359-4117.2003.01086.x"},{"key":"e_1_2_6_11_2","doi-asserted-by":"crossref","first-page":"4145","DOI":"10.4049\/jimmunol.158.9.4145","article-title":"Immunogenicity and immunopathogenicity of an autoimmune epitope are potentiated by increasing MHC binding through residue substitution","volume":"158","author":"Kozhich A T","year":"1997","journal-title":"J Immunol"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1002\/eji.200425413"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.169.9.5109"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0304-419X(03)00032-5"},{"key":"e_1_2_6_15_2","first-page":"5559","article-title":"Immunization of cancer patients with HER\u20102\/neu\u2010derived peptides demonstrating high\u2010affinity binding to multiple class II alleles","volume":"9","author":"Salazar L G","year":"2003","journal-title":"Clin Cancer Res"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.peptides.2003.12.004"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.0022-202X.2004.23417.x"},{"key":"e_1_2_6_18_2","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.10259"},{"key":"e_1_2_6_19_2","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.20310"},{"key":"e_1_2_6_20_2","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-03-3227"},{"key":"e_1_2_6_21_2","doi-asserted-by":"publisher","DOI":"10.1017\/S1462399401003362"},{"key":"e_1_2_6_22_2","doi-asserted-by":"publisher","DOI":"10.1007\/s002510050595"},{"key":"e_1_2_6_23_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/30.1.35"},{"key":"e_1_2_6_24_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.92.18.8125"},{"key":"e_1_2_6_25_2","doi-asserted-by":"crossref","first-page":"2213","DOI":"10.4049\/jimmunol.138.7.2213","article-title":"Prediction of immunodominant helper T cell antigenic sites from the primary sequence","volume":"138","author":"Margalit H","year":"1987","journal-title":"J Immunol"},{"key":"e_1_2_6_26_2","doi-asserted-by":"crossref","first-page":"204","DOI":"10.4049\/jimmunol.138.1.204","article-title":"Strong conformational propensities enhance T cell antigenicity","volume":"138","author":"Spouge J L","year":"1987","journal-title":"J Immunol"},{"key":"e_1_2_6_27_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.78.6.3824"},{"key":"e_1_2_6_28_2","doi-asserted-by":"publisher","DOI":"10.1016\/0076-6879(91)02013-Y"},{"key":"e_1_2_6_29_2","doi-asserted-by":"publisher","DOI":"10.1093\/intimm\/10.3.259"},{"key":"e_1_2_6_30_2","first-page":"109","article-title":"Transcending the structuralist paradigm in unmunology. 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