{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T00:04:19Z","timestamp":1761609859039},"reference-count":37,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2006,3,2]],"date-time":"2006-03-02T00:00:00Z","timestamp":1141257600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Experimental Dermatology"],"published-print":{"date-parts":[[2006,4]]},"abstract":"<jats:p><jats:bold>Abstract:\u2002<\/jats:bold> The 230\u2010kDa bullous pemphigoid antigen (BPAG1) is an integral component of hemidesmosomes. We have previously reported that interferon\u2010\u03b3 (IFN\u03b3) inhibits the transcription of the BPAG1 gene (<jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"#b1\">1<\/jats:ext-link>). Here we investigated the target sequences of IFN\u03b3\u2010signal transduction pathway in the BPAG1 promoter in epidermal keratinocytes. Transient transfections with 5\u2032\u2010deletion constructs of BPAG1 promoter\u2010luciferase reporter gene plasmids in cultured normal human epidermal keratinocytes (NHEK) allowed us to narrow the DNA region containing IFN\u03b3 inhibitory element (IGIE) to between \u22121 and \u221289, upstream from the transcription initiation site (+1). Homology search in this region identified a chimeric sequence, consisting of IFN\u2010stimulated responsive element (ISRE) with a partial 7\u2010bp sequence of IFN\u03b3 activation site (GAS), as identified in the guanylate\u2010binding protein (GBP) gene, inserted at its center. Functional analysis of IGIE, inserted in front of the heterologous thymidine kinase promoter, indicated that IGIE acts as a down\u2010regulatory element of the promoter through IFN\u03b3\u2010dependent signal pathway. Transient transfection studies with BPAG1 promoter\u2010reporter gene constructs containing mutated IGIE (with TT to GG transversions in the region of 5\u2032ISRE, GAS, and 3\u2032ISRE) demonstrated that disruption of the ISRE sequences, but not GAS, markedly suppressed the BPAG1 basal promoter activity and resulted in attenuated IFN\u03b3 response in keratinocytes. Our findings provide novel insight into the mechanism of IFN\u03b3 regulation in keratinocyte differentiation and proliferation.<\/jats:p>","DOI":"10.1111\/j.0906-6705.2006.00408.x","type":"journal-article","created":{"date-parts":[[2006,3,2]],"date-time":"2006-03-02T14:10:27Z","timestamp":1141308627000},"page":"308-314","source":"Crossref","is-referenced-by-count":9,"title":["Interferon\u2010\u03b3 down\u2010regulates expression of the 230\u2010kDa bullous pemphigoid antigen gene (BPAG1) in epidermal keratinocytes via novel chimeric sequences of ISRE and GAS"],"prefix":"10.1111","volume":"15","author":[{"given":"Takahide","family":"Kaneko","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Katsuto","family":"Tamai","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yasushi","family":"Matsuzaki","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takehiko","family":"Yamazaki","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hajime","family":"Nakano","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Atsushi","family":"Kon","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Isao","family":"Hashimoto","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Katsumi","family":"Hanada","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yasuhumi","family":"Kaneda","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jouni","family":"Uitto","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,3,2]]},"reference":[{"key":"e_1_2_7_2_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.270.13.7609"},{"key":"e_1_2_7_3_2","doi-asserted-by":"publisher","DOI":"10.1111\/1523-1747.ep12460914"},{"key":"e_1_2_7_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(81)90115-X"},{"key":"e_1_2_7_5_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI114036"},{"key":"e_1_2_7_6_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI116655"},{"key":"e_1_2_7_7_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1523-1747.1999.00546.x"},{"key":"e_1_2_7_8_2","doi-asserted-by":"crossref","first-page":"8825","DOI":"10.1016\/S0021-9258(18)52948-3","article-title":"Cloning of type XVII collagen. Complementary and genomic DNA sequences of mouse 180\u2010kDa bullous pemphigoid antigen (BPAG2) predict an interrupted collagenous domain, a transmembrane segment, and unusual features in the 5\u2032\u2010end of the gene and the 3\u2032\u2010untranslated region of the mRNA","volume":"268","author":"Li K","year":"1993","journal-title":"J Biol Chem"},{"key":"e_1_2_7_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0945-053X(98)00005-5"},{"key":"e_1_2_7_10_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0625.1998.tb00304.x"},{"key":"e_1_2_7_11_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.immunol.15.1.749"},{"key":"e_1_2_7_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)42719-6"},{"key":"e_1_2_7_13_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.8197455"},{"key":"e_1_2_7_14_2","doi-asserted-by":"publisher","DOI":"10.1101\/gad.2.4.383"},{"key":"e_1_2_7_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(89)90107-4"},{"key":"e_1_2_7_16_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.91.11.5046"},{"key":"e_1_2_7_17_2","first-page":"3711","article-title":"Expression of IFN regulatory factor family of proteins in lymphocytes","volume":"11996","author":"Nelson N","journal-title":"J Immunol"},{"key":"e_1_2_7_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1359-6101(00)00005-8"},{"key":"e_1_2_7_19_2","doi-asserted-by":"publisher","DOI":"10.1089\/jir.1997.17.121"},{"key":"e_1_2_7_20_2","doi-asserted-by":"crossref","first-page":"2016","DOI":"10.1016\/S0021-9258(17)42129-6","article-title":"Control of growth regulatory and differentiation\u2010specific genes in human epidermal keratinocytes by interferon gamma. 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