{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,12]],"date-time":"2025-09-12T18:37:01Z","timestamp":1757702221635},"reference-count":45,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2006,5,10]],"date-time":"2006-05-10T00:00:00Z","timestamp":1147219200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Experimental Dermatology"],"published-print":{"date-parts":[[2006,6]]},"abstract":"<jats:p><jats:bold>Abstract:\u2002<\/jats:bold> In skin, an evolving inflammatory or immune response is triggered by early release of a cytokine cascade into the extracellular space. Investigation of extracellular cytokine secretion <jats:italic>in situ<\/jats:italic> has been limited by low cut\u2010off filtering membranes and sample volume size and the inability to monitor changes in cytokine protein levels in real\u2010time <jats:italic>in situ<\/jats:italic>. Here, we combine for the first time the methods of intradermal microdialysis and antibody protein arraying to profile the early cascade of multiple cytokines in a complex inflammatory response exemplified by ultraviolet B (UVB)\u2010induced inflammation. We observed significant differences of the cytokine and growth factor responses after tissue injury by catheter placement and UVB\u2010induced inflammation. UVB irradiation initiates a rapid proinflammatory response followed by a mixed TH1\/TH2 response in which ultimately TH2 cytokines IL\u20104 and IL10 predominated after 24\u2003h. This most likely indicates the termination and self limitation of the inflammatory response. We conclude that the combination of dermal microdialysis and protein microarray offers a powerful tool to analyze in real\u2010time the complex and rapidly changing interstitial protein milieu during cutaneous inflammatory responses.<\/jats:p>","DOI":"10.1111\/j.0906-6705.2006.00429.x","type":"journal-article","created":{"date-parts":[[2006,5,10]],"date-time":"2006-05-10T13:12:42Z","timestamp":1147266762000},"page":"447-454","source":"Crossref","is-referenced-by-count":49,"title":["<i>In situ<\/i> profiling and quantification of cytokines released during ultraviolet B\u2010induced inflammation by combining dermal microdialysis and protein microarrays"],"prefix":"10.1111","volume":"15","author":[{"given":"Marco","family":"Averbeck","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Simone","family":"Beilharz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Matthias","family":"Bauer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carl","family":"Gebhardt","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexandra","family":"Hartmann","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Klaus","family":"Hochleitner","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Friederike","family":"Kauer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ursula","family":"Voith","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jan C.","family":"Simon","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christian","family":"Termeer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,5,10]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1159\/000074061"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2133.2001.144003650_146_3.x"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0091-6749(96)70313-5"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.168.8.3732"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00403-003-0387-6"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.taap.2003.08.019"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1615\/CritRevImmunol.v21.i4.40"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1039\/b211156j"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1043-4666(03)00224-2"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2133.2000.03410.x"},{"issue":"10","key":"e_1_2_6_12_2","doi-asserted-by":"crossref","first-page":"5192","DOI":"10.4049\/jimmunol.163.10.5192","article-title":"Differential modulation of human epidermal Langerhans cell maturation by ultraviolet B radiation","volume":"163","author":"Nakagawa S","year":"1999","journal-title":"J Immunol"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0625.1994.tb00263.x"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1751-1097.1996.tb09642.x"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-0625.2003.120208.x"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1017\/S0029665199001214"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.1159\/000424307"},{"key":"e_1_2_6_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0956-5663(97)00052-3"},{"key":"e_1_2_6_19_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.164.9.4752"},{"key":"e_1_2_6_20_2","doi-asserted-by":"publisher","DOI":"10.1006\/cimm.1994.1104"},{"key":"e_1_2_6_21_2","doi-asserted-by":"publisher","DOI":"10.1097\/00002480-199207000-00067"},{"issue":"3","key":"e_1_2_6_22_2","doi-asserted-by":"crossref","first-page":"247","DOI":"10.1042\/cs0850247","article-title":"Assessment of adipose tissue metabolism in man: comparison of Fick and microdialysis techniques","volume":"85","author":"Arner P","year":"1993","journal-title":"Clin Sci (Lond)"},{"key":"e_1_2_6_23_2","doi-asserted-by":"publisher","DOI":"10.1080\/00015559850135733"},{"key":"e_1_2_6_24_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0609.2004.00269.x"},{"key":"e_1_2_6_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jim.2004.04.018"},{"key":"e_1_2_6_26_2","doi-asserted-by":"publisher","DOI":"10.1097\/00024382-200304000-00001"},{"key":"e_1_2_6_27_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1523-1747.1999.00570.x"},{"key":"e_1_2_6_28_2","doi-asserted-by":"publisher","DOI":"10.1006\/cyto.1995.1004"},{"key":"e_1_2_6_29_2","doi-asserted-by":"publisher","DOI":"10.1111\/1523-1747.ep12292156"},{"key":"e_1_2_6_30_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.0022-202X.2004.23238.x"},{"key":"e_1_2_6_31_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00403-004-0522-z"},{"key":"e_1_2_6_32_2","doi-asserted-by":"publisher","DOI":"10.1080\/000155501750208100"},{"key":"e_1_2_6_33_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.0906-6705.2004.00246.x"},{"key":"e_1_2_6_34_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra041320"},{"key":"e_1_2_6_35_2","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-0625.11.s.1.3.x"},{"key":"e_1_2_6_36_2","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.1999.1455"},{"key":"e_1_2_6_37_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V93.5.1456.404a34_1456_1463"},{"key":"e_1_2_6_38_2","doi-asserted-by":"publisher","DOI":"10.1111\/1523-1747.ep12582809"},{"issue":"8","key":"e_1_2_6_39_2","doi-asserted-by":"crossref","first-page":"3783","DOI":"10.4049\/jimmunol.160.8.3783","article-title":"A cytokine cascade including prostaglandin E2, IL\u20104, and IL\u201010 is responsible for UV\u2010induced systemic immune suppression","volume":"160","author":"Shreedhar V","year":"1998","journal-title":"J Immunol"},{"key":"e_1_2_6_40_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0625.1997.tb00155.x"},{"key":"e_1_2_6_41_2","doi-asserted-by":"publisher","DOI":"10.1111\/1523-1747.ep12613446"},{"key":"e_1_2_6_42_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1440-1711.2001.01047.x"},{"issue":"3","key":"e_1_2_6_43_2","first-page":"149","article-title":"Angiogenic and angiostatic factors in the molecular control of angiogenesis","volume":"47","author":"Distler J H","year":"2003","journal-title":"Q J Nucl Med"},{"key":"e_1_2_6_44_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.0022-202X.2003.22101.x"},{"key":"e_1_2_6_45_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2133.2002.04669.x"},{"key":"e_1_2_6_46_2","doi-asserted-by":"publisher","DOI":"10.1185\/030079903125002216"}],"container-title":["Experimental Dermatology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.0906-6705.2006.00429.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.0906-6705.2006.00429.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,12]],"date-time":"2023-10-12T00:32:54Z","timestamp":1697070774000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.0906-6705.2006.00429.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2006,5,10]]},"references-count":45,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2006,6]]}},"alternative-id":["10.1111\/j.0906-6705.2006.00429.x"],"URL":"https:\/\/doi.org\/10.1111\/j.0906-6705.2006.00429.x","archive":["Portico"],"relation":{},"ISSN":["0906-6705","1600-0625"],"issn-type":[{"value":"0906-6705","type":"print"},{"value":"1600-0625","type":"electronic"}],"subject":[],"published":{"date-parts":[[2006,5,10]]}}}