{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T09:30:09Z","timestamp":1790501409030,"version":"4.1.0"},"reference-count":17,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2004,9,16]],"date-time":"2004-09-16T00:00:00Z","timestamp":1095292800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["resjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Insect Molecular Biology"],"published-print":{"date-parts":[[2004,10]]},"abstract":"Abstract<\/jats:title>\n \n Population reduction of mosquitoes is an effective method for controlling dengue fever and malaria transmission. Recent developments in control techniques include proposals to construct transgenic strains of mosquitoes carrying dominant, conditional\u2010lethal genes under the control of sex\u2010 and stage\u2010specific promoters. In order to identify such promoters, subtractive cDNA libraries derived from male and female pupal mRNA of the yellow fever mosquito,\n Aedes aegypti<\/jats:italic>\n , were constructed and screened. A cDNA clone, F49, corresponds to a gene expressed specifically in female pupae. Sequence analyses revealed that this gene belongs to the actin gene family, and therefore was designated\n Aedes Actin\u20104<\/jats:italic>\n (\n AeAct\u20104<\/jats:italic>\n ). Transcription analyses demonstrated that this gene is expressed predominantly in the indirect flight muscles and, to a lesser extent, the legs of developing female mosquitoes. The promoter of this gene may be a useful tool for developing conditional lethal strains of mosquitoes.\n <\/jats:p>","DOI":"10.1111\/j.0962-1075.2004.00519.x","type":"journal-article","created":{"date-parts":[[2004,9,16]],"date-time":"2004-09-16T11:09:44Z","timestamp":1095332984000},"page":"563-568","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":29,"title":["The\n AeAct\u20104<\/i>\n gene is expressed in the developing flight muscles of female\n Aedes aegypti<\/i>"],"prefix":"10.1111","volume":"13","author":[{"given":"D.","family":"Mu\u00f1oz","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Jimenez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"O.","family":"Marinotti","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A. A.","family":"James","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2004,9,16]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.140142697"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/jmedent\/33.6.955"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/21.11.2627"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0965-1748(02)00116-9"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.0962-1075.2001.00270.x"},{"key":"e_1_2_5_7_1","first-page":"47","article-title":"Studies on the contents of BHC and DDT in the organisms in the Pearl River estuary and adjacent sea areas","volume":"20","author":"Luo S.","year":"2001","journal-title":"Mar Sci Bull Haiyang Tongbao"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/15.6.2781"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/0305-0491(93)90222-Q"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-94-009-1535-0_2"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0269-7491(00)00085-3"},{"key":"e_1_2_5_12_1","volume-title":"Molecular Cloning, a Laboratory Manual","author":"Sambrook J.","year":"1989"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1080\/00039890009604039"},{"key":"e_1_2_5_14_1","volume-title":"Area\u2010Wide Control of Fruit Flies and Other Pests","author":"Tan K.H.","year":"2000"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.287.5462.2474"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/22.22.4673"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.0962-1075.2004.00481.x"},{"key":"e_1_2_5_18_1","first-page":"1","article-title":"Vector control for malaria and other mosquito\u2010borne diseases","volume":"857","author":"World Health Organization.","year":"1995","journal-title":"World Health Organ Tech Rep Ser"}],"container-title":["Insect Molecular Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.0962-1075.2004.00519.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/resjournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.0962-1075.2004.00519.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,11,2]],"date-time":"2025-11-02T12:56:35Z","timestamp":1762088195000},"score":1,"resource":{"primary":{"URL":"https:\/\/resjournals.onlinelibrary.wiley.com\/doi\/10.1111\/j.0962-1075.2004.00519.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2004,9,16]]},"references-count":17,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2004,10]]}},"alternative-id":["10.1111\/j.0962-1075.2004.00519.x"],"URL":"https:\/\/doi.org\/10.1111\/j.0962-1075.2004.00519.x","archive":["Portico"],"relation":{},"ISSN":["0962-1075","1365-2583"],"issn-type":[{"value":"0962-1075","type":"print"},{"value":"1365-2583","type":"electronic"}],"subject":[],"published":{"date-parts":[[2004,9,16]]},"assertion":[{"value":"2004-09-16","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}