{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,22]],"date-time":"2025-10-22T02:38:34Z","timestamp":1761100714115},"reference-count":16,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2004,7,16]],"date-time":"2004-07-16T00:00:00Z","timestamp":1089936000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Helicobacter"],"published-print":{"date-parts":[[2004,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p><jats:bold>Background.\u2002<\/jats:bold>  <jats:italic>Helicobacter pylori<\/jats:italic> is a causative agent of gastritis, and <jats:italic>H.\u00a0pylori<\/jats:italic> infection is thought to be correlated with iron\u2010deficiency anemia (IDA) at puberty. The <jats:italic>H.\u00a0pylori feoB<\/jats:italic> gene product, a high\u2010affinity ferrous iron transporter, plays a central role in iron acquisition and virulence. This study was undertaken to analyze <jats:italic>H.\u00a0pylori feoB<\/jats:italic> status according to clinical data, including antral gastritis with or without IDA.<\/jats:p><jats:p><jats:bold>Methods.\u2002<\/jats:bold> Fourteen <jats:italic>H.\u00a0pylori<\/jats:italic>\u2010positive patients aged from 10 to 18\u00a0years were categorized into subgroups based on the presence or absence of IDA. Eight patients were diagnosed as having IDA; the other six showed normal hematological findings. Genomic DNA was isolated from <jats:italic>H.\u00a0pylori<\/jats:italic> cultured from each gastric biopsy specimen. Five sets of primers were used for the PCR amplification of the <jats:italic>feoB<\/jats:italic> gene. Linking and sequencing of PCR products generated the <jats:italic>feoB<\/jats:italic> region, which was 1.93\u00a0kb in size. The <jats:italic>feoB<\/jats:italic> gene sequences of <jats:italic>H.\u00a0pylori<\/jats:italic> J99 and 26695 were compared with the clinical strains, and the sequences of <jats:italic>feoB<\/jats:italic> regions in the IDA (+) and (\u2013) groups were compared.<\/jats:p><jats:p><jats:bold>Results.\u2002<\/jats:bold> Sequence analysis of the complete coding region of the <jats:italic>feoB<\/jats:italic> gene revealed 16 sites of polymorphism or mutation. Among these, three polymorphisms (E\/T254A, I263V, and K511Q) were indigenous to the Korean clinical strains. Although statistically significant differences were observed at four sites (K127T, A273S\/P, I438V and I441T) between IDA (+) and (\u2013), the number of specimens was too low to assess the significance of the differences.<\/jats:p><jats:p><jats:bold>Conclusion.\u2002<\/jats:bold> The four polymorphisms of the <jats:italic>feoB<\/jats:italic> gene observed appear to be related to the clinical phenotype of IDA, but the relation is unclear because of the small number of strains studied. Further studies are required to confirm a correlation between IDA and <jats:italic>H.\u00a0pylori<\/jats:italic> infection.<\/jats:p>","DOI":"10.1111\/j.1083-4389.2004.00239.x","type":"journal-article","created":{"date-parts":[[2004,7,16]],"date-time":"2004-07-16T11:56:44Z","timestamp":1089979004000},"page":"330-334","source":"Crossref","is-referenced-by-count":21,"title":["Polymorphism of the <i>Helicobacter pylori feoB<\/i> Gene in Korea: a Possible Relation with Iron\u2010Deficiency Anemia?"],"prefix":"10.1111","volume":"9","author":[{"given":"Byung Ha","family":"Jeon","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoo Joung","family":"Oh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Na Gyong","family":"Lee","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yon Ho","family":"Choe","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2004,7,16]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1083-4389.2002.00073.x"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1097\/00005176-199901000-00027"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1097\/00005176-199308000-00018"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.3109\/00365529709025109"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.7326\/0003-4819-131-9-199911020-00006"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1136\/adc.82.2.136"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1523-5378.1999.98066.x"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1651-2227.2000.tb01208.x"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.3109\/00365529708996515"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.61.6.2694-2697.1993"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1523-5378.2001.00007.x"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.175.4.966-972.1993"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2000.01987.x"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1001\/jama.1997.03540360041028"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.242338299"},{"key":"e_1_2_6_17_2","volume-title":"Lehninger Principles of Biochemistry","author":"David LN","year":"2000"}],"container-title":["Helicobacter"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1083-4389.2004.00239.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1083-4389.2004.00239.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,12]],"date-time":"2023-10-12T17:33:45Z","timestamp":1697132025000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1083-4389.2004.00239.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2004,7,16]]},"references-count":16,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2004,8]]}},"alternative-id":["10.1111\/j.1083-4389.2004.00239.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1083-4389.2004.00239.x","archive":["Portico"],"relation":{},"ISSN":["1083-4389","1523-5378"],"issn-type":[{"value":"1083-4389","type":"print"},{"value":"1523-5378","type":"electronic"}],"subject":[],"published":{"date-parts":[[2004,7,16]]}}}