{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,25]],"date-time":"2025-09-25T16:53:09Z","timestamp":1758819189086},"reference-count":24,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2012,2,1]],"date-time":"2012-02-01T00:00:00Z","timestamp":1328054400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Fish Biology"],"published-print":{"date-parts":[[2012,3]]},"abstract":"<jats:p>The morphology of gametes and the fertilization biology of Japanese flounder <jats:italic>Paralichthys olivaceus<\/jats:italic> (Po), summer flounder <jats:italic>Paralichthys dentatus<\/jats:italic> (Pd) and their hybrids were examined. Multiple generations (two hybrids: Po\u2640\u00d7 Pd\u2642 (F1) and Pd\u2640\u00d7 Po\u2642; two backcrosses: F1\u2640\u00d7 Po\u2642 and F1\u2640\u00d7 Pd\u2642) were obtained by artificial insemination. Comparison of egg ultrastructure among Po, Pd and F1 showed the morphology of micropyle region and the distribution density of pores were species specific. There were <jats:italic>c<\/jats:italic>. 100\u2013200 accessory openings around the micropyle in Po, but not in Pd and F1. The zona radiata thickness and number of parallel bands were similar between F1 and Po, which were different from Pd. Comparison of spermatozoa ultrastructure revealed a close relationship between Po and Pd. Cytologically, the six crosses obeyed normal fertilization and cleavage processes, and only one male pronucleus was observed in a fertilized egg, indicating a monospermic fertilization pattern. Analysis of the time distribution from fertilization to first cleavage revealed an obvious delay at pronucleus fusion in the Pd \u00d7 Po cross. The delay might indicate some cytoplasmic\u2010nuclear incompatibility during the process of fertilization.<\/jats:p>","DOI":"10.1111\/j.1095-8649.2011.03146.x","type":"journal-article","created":{"date-parts":[[2012,2,1]],"date-time":"2012-02-01T18:05:00Z","timestamp":1328119500000},"page":"473-485","source":"Crossref","is-referenced-by-count":5,"title":["Germ cells and fertilization differences among Japanese flounder <i>Paralichthys olivaceus<\/i>, summer flounder <i>Paralichthys dentatus<\/i> and their first and second generations"],"prefix":"10.1111","volume":"80","author":[{"given":"J.","family":"Sui","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D. Y.","family":"Ma","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Q. 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