{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,30]],"date-time":"2023-10-30T08:44:25Z","timestamp":1698655465116},"reference-count":13,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2015,4,9]],"date-time":"2015-04-09T00:00:00Z","timestamp":1428537600000},"content-version":"vor","delay-in-days":5486,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Dermatology"],"published-print":{"date-parts":[[2000,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>A polymerase chain reaction (PCR)\u2010based strategy has been developed for analysis of clonal rearrangement of the T\u2010cell receptor \u03b3 gene (TCR\u03b3) and was shown to be useful for detection of clonal T\u2010cell populations. In this study, we performed PCR combined with denaturing gradient gel electrophoresis (DGGE) on fresh frozen biopsy samples from 16 patients with cutaneous T\u2010lymphproliferative diseases in whom a definite diagnosis was difficult to make on morphological and immunohistochemical grounds alone. Ages of the patients at biopsy ranged from 28 to 81 (median 62) years, and the subjects consisted of 8 men and 8 women. They presented with erythema on the extremities in 5 cases, trunk in 7, buttock in 2, and papules on the trunk and face in one case each. Clonal rearrangement of TCR\u03b3 was observed in 3 of 16 cases. Clinical diagnoses of these three cases were mycosis fungoides, cutaneous invasion of adult T\u2010cell leukemia (ATL), and large granular lymphocytic leukemia (LGL) of T\u2010cell type, respectively, but they were histologically difficult to differentiate from reactive cutaneous T\u2010cell proliferation. The skin lesions of the LGL case worsened, and this patient died two years after biopsy. Another patient with suspected mycosis fungoides in the plaque stage died due to dissemination of tumors 22 months after biopsy. The remaining one patient with ATL survived with cutaneous lesions for over four years. Clonality was not demonstrated in the remaining 13 cases, and their clinical courses were favorable. These findings showed that demonstration of clonal TCR\u03b3 gene rearrangement using the PCR\u2010DGGE method is very helpful for diagnosis of cutaneous T\u2010cell neoplasms.<\/jats:p>","DOI":"10.1111\/j.1346-8138.2000.tb02158.x","type":"journal-article","created":{"date-parts":[[2015,4,10]],"date-time":"2015-04-10T06:14:02Z","timestamp":1428646442000},"page":"238-243","source":"Crossref","is-referenced-by-count":4,"title":["Analysis of T\u2010Cell Antigen Receptor \u03b3 Chain Gene Rearrangement by Polymerase Chain Reaction in Combination with Denaturing Gradient Gel Electrophoresis in the Differential Diagnosis of Cutaneous T\u2010Lymphoproliferative Diseases"],"prefix":"10.1111","volume":"27","author":[{"given":"Tomohiko","family":"Nagasawa","sequence":"first","affiliation":[{"name":"Departments of Pathology Osaka University Medical School  Suita Oasaka Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shin\u2010ichi","family":"Nakatsuka","sequence":"additional","affiliation":[{"name":"Departments of Pathology Osaka University Medical School  Suita Oasaka Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hideaki","family":"Miwa","sequence":"additional","affiliation":[{"name":"Departments of Pathology Osaka University Medical School  Suita Oasaka Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiroyuki","family":"Kanno","sequence":"additional","affiliation":[{"name":"Departments of Pathology Osaka University Medical School  Suita Oasaka Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Satoshi","family":"Itami","sequence":"additional","affiliation":[{"name":"Departments of Dermatology Osaka University Medical School  Suita Oasaka 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