{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,11,21]],"date-time":"2023-11-21T05:04:27Z","timestamp":1700543067415},"reference-count":13,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2013,11,14]],"date-time":"2013-11-14T00:00:00Z","timestamp":1384387200000},"content-version":"vor","delay-in-days":4975,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microbiology and Immunology"],"published-print":{"date-parts":[[2000,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>We screened a phage library of <jats:italic>Rickettsia typhi<\/jats:italic> with a polyclonal antiserum to clone genes which encode immunogenic proteins of <jats:italic>R. typhi<\/jats:italic>. Among several clones obtained, one clone codes for a 466\u2010amino\u2010acid protein similar to the heat\u2010shock protein, HtrA. The deduced rickettsial HtrA contains a putative signal peptide sequence at the N\u2010terminus, a serine protease\u2010like domain, and two PDZ domains. The recombinant protein of rickettsial HtrA reacted with sera from patients with murine typhus and tsutsugamushi disease. We suggest that this gene and its recombinant protein would be valuable for the immunologic diagnosis of rickettsial diseases.<\/jats:p>","DOI":"10.1111\/j.1348-0421.2000.tb02495.x","type":"journal-article","created":{"date-parts":[[2013,11,14]],"date-time":"2013-11-14T15:58:30Z","timestamp":1384444710000},"page":"275-278","source":"Crossref","is-referenced-by-count":2,"title":["Cloning and Sequencing of the Gene Encoding the Candidate HtrA of <i>Rickettsia typhi<\/i>"],"prefix":"10.1111","volume":"44","author":[{"given":"Jong\u2010hyun","family":"Kim","sequence":"first","affiliation":[{"name":"Department of Microbiology College of Medicine, Kon\u2010Kuk University  Choongju 380\u2010701 South Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Myong\u2010joon","family":"Hahn","sequence":"additional","affiliation":[{"name":"Basic Medical Sciences Sungkyunkwan University School of Medicine  Suwon 440\u2010746 Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2013,11,14]]},"reference":[{"key":"e_1_2_2_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2836(05)80360-2"},{"key":"e_1_2_2_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/24094"},{"key":"e_1_2_2_4_1","doi-asserted-by":"crossref","first-page":"276","DOI":"10.1128\/iai.31.1.276-288.1981","article-title":"Partial purification and characterization of the major species\u2010specific protein antigens of Rickettsia typhi and Rickettsia prowazekii identified by rocket immunoelectrophoresis","volume":"32","author":"Dasch G.A.","year":"1981","journal-title":"Infect. Immun."},{"key":"e_1_2_2_5_1","doi-asserted-by":"publisher","DOI":"10.1128\/iai.64.11.4838-4841.1996"},{"key":"e_1_2_2_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1119(93)90237-W"},{"key":"e_1_2_2_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1991.tb02122.x"},{"key":"e_1_2_2_8_1","doi-asserted-by":"publisher","DOI":"10.1128\/jb.171.3.1574-1584.1989"},{"key":"e_1_2_2_9_1","doi-asserted-by":"publisher","DOI":"10.1128\/jb.172.4.1791-1797.1990"},{"key":"e_1_2_2_10_1","doi-asserted-by":"crossref","first-page":"899","DOI":"10.1128\/IAI.66.3.899-906.1998","article-title":"The Haemophilus influenzae HtrA protein is a protective antigen","volume":"66","author":"Loosmore S.M.","year":"1998","journal-title":"Infect. Immun."},{"key":"e_1_2_2_11_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.1997.5601928.x"},{"key":"e_1_2_2_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/pro.5560060225"},{"key":"e_1_2_2_13_1","doi-asserted-by":"crossref","first-page":"1000","DOI":"10.1128\/iai.62.3.1000-1007.1994","article-title":"Identification of an immunoreactive Brucella abortus HtrA stress response protein homolog","volume":"62","author":"Roop R.M.","year":"1994","journal-title":"Infect. Immun."},{"key":"e_1_2_2_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-291X(05)80245-1"}],"container-title":["Microbiology and Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1348-0421.2000.tb02495.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1348-0421.2000.tb02495.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,21]],"date-time":"2023-11-21T01:32:44Z","timestamp":1700530364000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1348-0421.2000.tb02495.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2000,4]]},"references-count":13,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2000,4]]}},"alternative-id":["10.1111\/j.1348-0421.2000.tb02495.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1348-0421.2000.tb02495.x","archive":["Portico"],"relation":{},"ISSN":["0385-5600","1348-0421"],"issn-type":[{"value":"0385-5600","type":"print"},{"value":"1348-0421","type":"electronic"}],"subject":[],"published":{"date-parts":[[2000,4]]}}}