{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,9,4]],"date-time":"2024-09-04T21:00:09Z","timestamp":1725483609069},"reference-count":33,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2013,11,14]],"date-time":"2013-11-14T00:00:00Z","timestamp":1384387200000},"content-version":"vor","delay-in-days":3697,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microbiology and Immunology"],"published-print":{"date-parts":[[2003,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Genetic background determines the histological features of experimental immune\u2010mediated blepharoconjunctivitis (EC) in rats, which is a model for human allergic conjunctivitis (AC). A great number of lymphocytes predominate in EC of Lewis rats, while less lymphocytes and more eosinophils are present in that of Brown Norway (BN) rats. Although this difference could be attributed to their systemic Th1\/Th2 dominancy, it remains unclear whether some regulatory mechanisms may exist in the inflammatory site in the conjunctiva. Here, we aim to investigate this hypothesis by comparing the expression levels of inflammatory mediators in the conjunctiva between the two strains. EC was induced in Lewis and BN rats by transfer of ovalbumin (OVA)\u2010specific CD4<jats:sup>+<\/jats:sup> T\u2010cell lines followed by eye drops of OVA as antigen challenge, and then was clinically and histologically evaluated. Reverse\u2010transcription (RT)\u2010PCR was performed to compare the expressions of cytokines and cytokine receptors (Rs) in conjunctivas of both strains of rats either with or without EC. To confirm the biological significance of interferon (IFN)\u2010\u03b3 R expression, phosphorylation of signal transducers and activators of transcription (STAT)\u20101 was examined in the conjunctivas, followed by subconjunctival injection of IFN\u2010\u03b3. BN T cells contained interleukin (IL)\u20104 and IFN\u2010\u03b3, while Lewis T cells expressed no IL\u20104. Transfer of those cells induced more severe EC in Lewis rats. RT\u2010PCR using naive conjunctivas detected more IL\u20104, IFN\u2010\u03b3, and IFN\u2010\u03b3R \u03b2\u2010chain RNA expression in BN rats. After the EC induction, BN rats expressed significantly higher amounts of IFN\u2010\u03b3R \u03b2\u2010chain, and upregulation of interferon regulatory factor (IRF)\u20101 was observed. Phosphorylation of STAT\u20101 was more remarkable in BN rats. The findings demonstrate differential expression of IFN\u2010\u03b3R and signaling through IFN\u2010\u03b3 in the conjunctiva between the two strains. This may be due to differences in histopathological character between the two strains.<\/jats:p>","DOI":"10.1111\/j.1348-0421.2003.tb03436.x","type":"journal-article","created":{"date-parts":[[2013,11,14]],"date-time":"2013-11-14T15:27:04Z","timestamp":1384442824000},"page":"785-796","source":"Crossref","is-referenced-by-count":5,"title":["Differential Expression and Signaling of IFN\u2010\u03b3 in the Conjunctiva between Lewis and Brown Norway Rats"],"prefix":"10.1111","volume":"47","author":[{"given":"Atsuki","family":"Fukushima","sequence":"first","affiliation":[{"name":"Laboratory of Immunology, Department of Ophthalmology Kochi Medical School  Nankoku Kochi 783\u20108505 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Akemi","family":"Ozaki","sequence":"additional","affiliation":[{"name":"Laboratory of Immunology, Department of Ophthalmology Kochi Medical School  Nankoku Kochi 783\u20108505 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kazuyo","family":"Fukata","sequence":"additional","affiliation":[{"name":"Laboratory of Immunology, Department of Ophthalmology Kochi Medical School  Nankoku Kochi 783\u20108505 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hisayuki","family":"Ueno","sequence":"additional","affiliation":[{"name":"Laboratory of Immunology, Department of Ophthalmology Kochi Medical School  Nankoku Kochi 783\u20108505 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2013,11,14]]},"reference":[{"key":"e_1_2_2_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(88)90050-5"},{"key":"e_1_2_2_3_1","first-page":"1646","article-title":"Involvement of IFN\u2010\u03b3 receptor\u2010mediated signaling in pathology and anti\u2010malarial immunity induced by Plasmodium berghei infection. 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