{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,15]],"date-time":"2025-10-15T04:18:36Z","timestamp":1760501916503},"reference-count":50,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2012,2,13]],"date-time":"2012-02-13T00:00:00Z","timestamp":1329091200000},"content-version":"vor","delay-in-days":43,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microbiology and Immunology"],"published-print":{"date-parts":[[2012,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p><jats:italic>Helicobacter pylori<\/jats:italic> (H.p) colonizes human gastric mucosa and causes gastric and duodenal ulcer disease or gastric cancer. Various H.p compounds may modulate the host immune response in regards to tolerance of the infection or disease development. The aim of this study was to determine whether H.p lipopolysaccharide (LPS) and glycine acid extract antigens (GE) or <jats:italic>E. coli<\/jats:italic> LPS influence the cytotoxic activity of peripheral blood lymphocytes from H.p infected \u2013 H.p (+) or uninfected \u2013 H.p (\u2212) individuals, in the presence or absence of exogenous interleukin (IL)12. Individual H.p status was defined by the urea breath test. Lymphocytes, stimulated or not with H.p, and control antigens, with or without IL\u201012, were used as effector cells and epithelial HeLa cells as targets. The cytotoxicity of lymphocytes was expressed as the percentage of dead target cells unable to reduce tetrazolium salt. The supernatants from HeLa\/lymphocyte cultures were used for detection of the cellular cytotoxicity markers granzyme B and caspase 8. The natural cytotoxic activity of lymphocytes from H.p (+) was less than that of H.p (\u2212) donors. This may have been due to fewer natural killer cells of CD3<jats:sup>\u2212<\/jats:sup>CD56<jats:sup>+<\/jats:sup>Nkp46<jats:sup>+<\/jats:sup> phenotype in H.p (+) in comparison to H.p (\u2212) subjects. H.p GE and standard <jats:italic>E. coli<\/jats:italic> LPS enhanced the cytotoxicity of lymphocytes towards target cells whereas H.p LPS downregulated this activity. The decrease in lymphocyte cytotoxicity in response to H.p LPS correlated with a lack of IL\u20102 and IL\u201012 production, inhibition of interferon\u2010\u03b3 production, and low IL\u201010 secretion by mononuclear leukocytes. IL\u201012 significantly enhanced the natural as well as H.p LPS and H.p GE driven cytotoxic capacity of lymphocytes. In conclusion, H.p LPS may negatively modulate natural cytotoxic activity and cytokine secretion by immunocompetent cells and thus be involved in the maintenance of infection and development of gastric pathologies.<\/jats:p>","DOI":"10.1111\/j.1348-0421.2011.00399.x","type":"journal-article","created":{"date-parts":[[2011,11,1]],"date-time":"2011-11-01T09:32:04Z","timestamp":1320139924000},"page":"62-75","source":"Crossref","is-referenced-by-count":9,"title":["<i>Helicobacter pylori<\/i> antigens as potential modulators of lymphocytes\u2019 cytotoxic activity"],"prefix":"10.1111","volume":"56","author":[{"given":"Karolina","family":"Rudnicka","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marcin","family":"W\u0142odarczyk","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anthony P.","family":"Moran","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tomasz","family":"Rechci\u0144ski","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eliza","family":"Miszczyk","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Agnieszka","family":"Matusiak","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ewelina","family":"Szcz\u0119sna","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria","family":"Walencka","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wies\u0142awa","family":"Rudnicka","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Magdalena","family":"Chmiela","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2012,2,13]]},"reference":[{"key":"e_1_2_8_2_2","doi-asserted-by":"crossref","first-page":"1311","DOI":"10.1016\/S0140-6736(84)91816-6","article-title":"Unidentified curved bacilli in the stomach of patients with gastritis and peptic ulceration","volume":"341","author":"Marshall B.J.","year":"1984","journal-title":"Lancet"},{"key":"e_1_2_8_3_2","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2009.01.073"},{"key":"e_1_2_8_4_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1478-405X.2006.00422.x"},{"key":"e_1_2_8_5_2","doi-asserted-by":"publisher","DOI":"10.1007\/s005350050373"},{"key":"e_1_2_8_6_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.165.2.1022"},{"key":"e_1_2_8_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1471-4906(01)02060-9"},{"key":"e_1_2_8_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0928-8244(03)00028-2"},{"key":"e_1_2_8_9_2","doi-asserted-by":"crossref","first-page":"4729","DOI":"10.4049\/jimmunol.156.12.4729","article-title":"Helicobacter\u2010specific cell\u2010mediated immune responses display a predominant Th1 phenotype and promote a delayed\u2010type hypersensitivity response in the stomach of mice","volume":"156","author":"Mohammadi M.S.","year":"1996","journal-title":"J Immunol"},{"key":"e_1_2_8_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.clim.2005.09.011"},{"key":"e_1_2_8_11_2","doi-asserted-by":"publisher","DOI":"10.1124\/pr.55.2.4"},{"key":"e_1_2_8_12_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.gene.6364135"},{"key":"e_1_2_8_13_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1523-5378.2008.00641.x"},{"key":"e_1_2_8_14_2","doi-asserted-by":"publisher","DOI":"10.1159\/000286238"},{"key":"e_1_2_8_15_2","doi-asserted-by":"publisher","DOI":"10.1002\/eji.200838882"},{"key":"e_1_2_8_16_2","first-page":"3012","article-title":"Contact of lymphocytes with Helicobacter pylori augments natural killer cell activity and induces production of IFN\u2010 \u03b3. 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