{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T21:18:28Z","timestamp":1760217508887},"reference-count":57,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2012,4,16]],"date-time":"2012-04-16T00:00:00Z","timestamp":1334534400000},"content-version":"vor","delay-in-days":15,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Microbiology and Immunology"],"published-print":{"date-parts":[[2012,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>We investigated the correlation between the SOD activity of <jats:italic>Helicobacter pylori<\/jats:italic> (<jats:italic>H. pylori<\/jats:italic>) and gastroduodenal diseases and the characteristics of strains exposed to oxidative stress. Two sequenced strains, 26695 and J99, and clinical isolates from 156 Japanese patients with gastroduodenal diseases such as gastric cancer (<jats:italic>n<\/jats:italic>= 59) and non\u2010cancer (<jats:italic>n<\/jats:italic>= 97) were used. SOD activities of all 158 isolates were measured and were divided into three groups: high\u2010SOD activity (&gt;0.22, <jats:italic>n<\/jats:italic>= 2), moderate\u2010SOD activity (0.15\u2266\u22660.22, <jats:italic>n<\/jats:italic>= 16) and low\u2010SOD activity (&lt;0.15, <jats:italic>n<\/jats:italic>= 140). Expressions of <jats:italic>H. pylori<\/jats:italic> Fe\u2010SOD were examined by western blotting with anti\u2010<jats:italic>H. pylori<\/jats:italic> Fe\u2010SOD antibody prepared inhouse, and the profiles of Fe\u2010SOD activity were investigated by zymogram with activity staining in native\u2010PAGE. The characteristics of strains from high\u2010SOD and low\u2010SOD groups were examined under oxidative stress by paraquat. The average of <jats:italic>H. pylori<\/jats:italic> SOD activity was significantly higher in the cancer group than in the non\u2010cancer group (<jats:italic>P<\/jats:italic> &lt; 0.05). However, irrespective of SOD activity level, the amount of Fe\u2010SOD expressed was variable among individual strains. Zymogram revealed a single band in moderate\u2010SOD and low\u2010SOD strains, but multiple bands in high\u2010SOD strains were observed. These bands were confirmed as <jats:italic>H. pylori<\/jats:italic> Fe\u2010SOD. Under oxidative stress with paraquat, low\u2010SOD strains were drastically eliminated without inducible SOD activity; however, high\u2010SOD strains were still viable with increased SOD activity. This study is the first to exhibit the characteristics of high\u2010SOD activity strains representing multiple bands in zymograms and the correlation between <jats:italic>H. pylori<\/jats:italic> SOD activity and gastric cancer.<\/jats:p>","DOI":"10.1111\/j.1348-0421.2012.00433.x","type":"journal-article","created":{"date-parts":[[2012,1,30]],"date-time":"2012-01-30T19:39:52Z","timestamp":1327952392000},"page":"262-272","source":"Crossref","is-referenced-by-count":15,"title":["Superoxide dismutase activity of <i>Helicobacter pylori per se<\/i> from 158 clinical isolates and the 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