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In the current study, whether stationary phase and stress response sigma factor RpoS and BpsI\u2010mediated quorum sensing (QS) system co\u2010regulate its target genes was investigated. Positive regulation of RpoS on <jats:italic>bpsI<\/jats:italic> expression and autoinducer production in stationary phase, but not on <jats:italic>fliC<\/jats:italic> and <jats:italic>ppk<\/jats:italic> expression, was observed. In contrast, expression of <jats:italic>rpoS<\/jats:italic> was not affected by deletion of <jats:italic>bpsI<\/jats:italic>. The present results also indicate that production of extracellular protease and siderophore, two QS\u2010controlled exo products, is regulated via RpoS supporting the previously known inhibitory role of QS on those two factors. Proteomic analysis revealed that expression of 74 protein spots representing 60 genes is controlled by QS. Of those, 45 genes are co\u2010regulated by both RpoS and QS, and regulation of genes involved in transcription and translation is favored by QS. Taken together, our findings indicate major target genes expression in stationary phase that is influenced by hierarchical control of RpoS over QS. We propose that this regulation may play an important role in the pathogenicity of <jats:italic>B. pseudomallei<\/jats:italic>.<\/jats:p>","DOI":"10.1111\/j.1348-0421.2012.00447.x","type":"journal-article","created":{"date-parts":[[2012,3,5]],"date-time":"2012-03-05T15:49:15Z","timestamp":1330962555000},"page":"281-294","source":"Crossref","is-referenced-by-count":30,"title":["Regulation of a quorum sensing system by stationary phase sigma factor RpoS and their co\u2010regulation of target genes\u2028 in <i>Burkholderia 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