{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,5,13]],"date-time":"2024-05-13T11:27:41Z","timestamp":1715599661742},"reference-count":24,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2006,1,25]],"date-time":"2006-01-25T00:00:00Z","timestamp":1138147200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cancer Science"],"published-print":{"date-parts":[[2006,2]]},"abstract":"<jats:p>Peptide\u2010MHC tetramers have been engineered to allow accurate detection of antigen\u2010specific cytotoxic C lymphocytes (CTL) by flow cytometry. Here, we propose a novel use for peptide\u2010MHC tetramers in the specific and sensitive analysis of the cytotoxic function of antigen\u2010specific CTL by blocking MHC\u2010restricted antigen\u2010specific cytotoxicity. We found that pretreatment of ovalbumin (OVA)\u2010specific CD8<jats:sup>+<\/jats:sup> CTL (OT\u20101 CTL), derived from OT\u20101 T\u2010cell receptor (TCR)\u2010transgenic mice, with OVA<jats:sub>257\u2212264<\/jats:sub> peptide\u2010H\u20102K<jats:sup>b<\/jats:sup> tetramer caused a marked inhibition of the cytotoxicity against OVA\u2010expressing EG\u20107 tumor cells. OVA<jats:sub>257\u2212264<\/jats:sub> peptide\u2010H\u20102K<jats:sup>b<\/jats:sup> tetramer did not block the cytotoxicity mediated by 2C mouse (H\u20102<jats:sup>b<\/jats:sup>)\u2010derived CD8<jats:sup>+<\/jats:sup> CTL, which recognize allo (H\u20102L<jats:sup>d<\/jats:sup>) antigens. Moreover, OT\u2010I CTL activity was not inhibited by an irrelevant HBV<jats:sub>208\u2212216<\/jats:sub> peptide\u2010H\u20102K<jats:sup>b<\/jats:sup> tetramer. These results indicate that the blocking of CTL activity with peptide\u2010MHC tetramer was caused by interference with the interaction between the TCR and H\u20102K<jats:sup>b<\/jats:sup>\u2010OVA<jats:sub>257\u2212264<\/jats:sub> peptide complex, but not with the CD8\u2010MHC class I interaction. The blocking activity of OVA<jats:sub>257\u2212264<\/jats:sub> peptide\u2010H\u20102K<jats:sup>b<\/jats:sup> tetramer was reversible because OT\u2010I CTL pretreated with the tetramer recovered their cytotoxicity after culturing with interleukin\u20102 for 24\u00a0h. The same results were also demonstrated in freshly isolated, <jats:italic>in\u00a0vivo<\/jats:italic>\u2010primed OT\u20101 CTL sorted by the tetramer. These results demonstrate that peptide\u2010MHC tetramer is a useful tool for defining MHC\u2010restricted antigen\u2010specific CTL function. Moreover, our finding implies that the measurement of CTL activity immediately after tetramer\u2010guided sorting is not a suitable method for evaluating the function of <jats:italic>in\u00a0vivo<\/jats:italic>\u2010induced tetramer\u2010positive CTL. We believe that the tetramer\u2010blocking assay presented here will be useful for functionally monitor the induction of MHC\u2010restricted antigen\u2010specific CTL during vaccination therapy against tumor and infectious diseases. (<jats:italic>Cancer Sci<\/jats:italic> 2006; 97: 148\u2003\u2013154)<\/jats:p>","DOI":"10.1111\/j.1349-7006.2006.00149.x","type":"journal-article","created":{"date-parts":[[2006,1,25]],"date-time":"2006-01-25T11:32:25Z","timestamp":1138188745000},"page":"148-154","source":"Crossref","is-referenced-by-count":13,"title":["Tetramer\u2010blocking assay for defining antigen\u2010specific cytotoxic T lymphocytes using peptide\u2010MHC tetramer"],"prefix":"10.1111","volume":"97","author":[{"given":"Hiroshi","family":"Yokouchi","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kenji","family":"Chamoto","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daiko","family":"Wakita","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daisuke","family":"Noguchi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Koichi","family":"Yamazaki","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hirotoshi","family":"Dosaka\u2010Akita","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masaharu","family":"Nishimura","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiroaki","family":"Ikeda","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takashi","family":"Nishimura","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,1,25]]},"reference":[{"key":"e_1_2_7_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0167-5699(98)01390-5"},{"key":"e_1_2_7_3_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.274.5284.94"},{"key":"e_1_2_7_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1074-7613(00)80599-3"},{"key":"e_1_2_7_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/9525"},{"key":"e_1_2_7_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0952-7915(00)00196-5"},{"key":"e_1_2_7_7_2","first-page":"3521","article-title":"Frequencies 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