{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,22]],"date-time":"2026-07-22T02:41:22Z","timestamp":1784688082070,"version":"3.55.0"},"reference-count":33,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2007,7,19]],"date-time":"2007-07-19T00:00:00Z","timestamp":1184803200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cancer Science"],"published-print":{"date-parts":[[2007,9]]},"abstract":"<jats:p>The authors investigated the DNA methylation patterns of the <jats:italic>E\u2010cadherin<\/jats:italic>,<jats:italic> Connexin 26<\/jats:italic> (<jats:italic>Cx26<\/jats:italic>), <jats:italic>Rassf1a<\/jats:italic> and <jats:italic>c\u2010fos<\/jats:italic> genes in the early phase of rat hepatocarcinogenesis induced by a choline\u2010deficient L\u2010amino acid\u2010defined (CDAA) diet. Six\u2010week\u2010old F344 male rats were continuously fed with the CDAA diet, and three animals were then killed at each of 4 and 8\u00a0days and 3\u00a0weeks. Genomic DNA was extracted from livers for assessment of methylation status in the 5\u2032 upstream regions of <jats:italic>E\u2010cadherin<\/jats:italic>, <jats:italic>Cx26<\/jats:italic>, <jats:italic>Rassf1a<\/jats:italic> and <jats:italic>c\u2010fos<\/jats:italic> genes by bisulfite sequencing, compared with normal livers. The livers of rats fed the CDAA diet for 4 and 8\u00a0days and 3\u00a0weeks were methylated in <jats:italic>E\u2010cadherin<\/jats:italic>, <jats:italic>Cx26<\/jats:italic> and <jats:italic>Rassf1a<\/jats:italic> genes, while normal livers were all unmethylated. In contrast, normal livers were highly methylated in <jats:italic>c\u2010fos<\/jats:italic> gene. Although the livers at 4\u00a0days were weakly methylated, those at 8\u00a0days and 3\u00a0weeks were markedly unmethylated. Methylation patterns of CpG sites in <jats:italic>E\u2010cadherin<\/jats:italic>, <jats:italic>Cx26<\/jats:italic> and <jats:italic>Rassf1a<\/jats:italic> were sparse and the methylation was not associated with gene repression. These results indicate that gene\u2010specific DNA methylation patterns were found in livers of rats after short\u2010term feeding of the CDAA diet, suggesting gene\u2010specific hypermethylation might be involved in the early phase of rat hepatocarcinogenesis induced by the CDAA diet. (<jats:italic>Cancer Sci<\/jats:italic> 2007; 98: 1318\u20131322)<\/jats:p>","DOI":"10.1111\/j.1349-7006.2007.00564.x","type":"journal-article","created":{"date-parts":[[2007,7,19]],"date-time":"2007-07-19T08:48:38Z","timestamp":1184834918000},"page":"1318-1322","source":"Crossref","is-referenced-by-count":32,"title":["Disturbance of DNA methylation patterns in the early phase of hepatocarcinogenesis induced by a choline\u2010deficient L\u2010amino acid\u2010defined diet in 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