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We previously described a system in which hyperthermia was induced using thermosensitive ferromagnetic particles (FMP) with a Curie temperature (Tc\u00a0=\u00a043\u02daC) low enough to mediate automatic temperature control, and demonstrated its antitumor effect in a mouse melanoma model. In the present study, we examined the antitumor effects of combining a Hsp90 inhibitor (geldanamycin; GA) with FMP\u2010mediated hyperthermia. In cultured B16 melanoma cells, GA exerted an antitumor effect by increasing the cells\u2019 susceptibility to hyperthermia and reducing expression of Akt. In an <jats:italic>in\u00a0vivo<\/jats:italic> study, melanoma cells were subcutaneously injected into the backs of C57BL\/6 mice. FMP were then injected into the resultant tumors, and the mice were divided into four groups: group I, no treatment (control); group II, one hyperthermia treatment; group III, GA alone; and group IV, GA with hyperthermia. When exposed to a magnetic field, the temperature of tissues containing FMP increased and stabilized at the Tc. In group IV, complete regression of tumors was observed in five of nine mice (56%), whereas no tumor regression was seen in groups I\u2013III. Our findings suggest that inhibition of Hsp90 with hyperthermia increases its antitumor effect. Thus, the combination of FMP\u2010mediated, self\u2010regulating hyperthermia with Hsp90 inhibition has important implications for the treatment of cancer. 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