{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T01:43:14Z","timestamp":1791164594748,"version":"4.1.0"},"reference-count":36,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2010,3,15]],"date-time":"2010-03-15T00:00:00Z","timestamp":1268611200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2010,5,19]],"date-time":"2010-05-19T00:00:00Z","timestamp":1274227200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cancer Science"],"published-print":{"date-parts":[[2010,6]]},"abstract":"<jats:p>\n                    <jats:italic>Bcl11b<\/jats:italic>\n                    encodes a zinc\u2010finger transcription factor and functions as a haploinsufficient tumor suppressor gene.\n                    <jats:italic>Bcl11b<\/jats:italic>\n                    <jats:sup>KO\/KO<\/jats:sup>\n                    mice exhibit differentiation arrest of thymocytes during \u03b2\u2010selection as has been observed with other mouse models involving knockouts of genes in the Wnt\/\u03b2\u2010catenin signaling pathway. Recurrent chromosomal rearrangement at the\n                    <jats:italic>BCL11B<\/jats:italic>\n                    locus occurs in human T\u2010cell leukemias, but it is not clear how such rearrangement would contribute to lymphomagenesis. To address this issue, we studied clonal cell growth, cell number, and differentiation of thymocytes in\n                    <jats:italic>Bcl11b<\/jats:italic>\n                    <jats:sup>KO\/+<\/jats:sup>\n                    mice at different time points following \u03b3\u2010irradiation. Analysis of D\u2010J rearrangement at the T cell receptor \u03b2\u2010chain (\n                    <jats:italic>TCR\u03b2<\/jats:italic>\n                    ) locus and cell surface markers by flow cytometry revealed two distinct populations of clonally growing thymocytes. In one population, thymocytes share a common D\u2010J rearrangement but retain the capacity to differentiate. In contrast, thymocytes in the second population have lost their ability to differentiate. Since the capacity to self renew and differentiate into multiple cell lineages are fundamental properties of adult stem cells, the differentiation competent population of thymocytes that we have isolated could potentially function as cancer stem cells. We also demonstrate increased expression of \u03b2\u2010catenin, a well\u2010known oncogenic protein, in\n                    <jats:italic>Bcl11b<\/jats:italic>\n                    <jats:sup>KO\/+<\/jats:sup>\n                    thymocytes. Collectively, the\n                    <jats:italic>Bcl11b<\/jats:italic>\n                    <jats:sup>KO\/+<\/jats:sup>\n                    genotype contributes to clonal expansion and differentiation arrest in part through an increase in the level of \u03b2\u2010catenin. (\n                    <jats:italic>Cancer Sci<\/jats:italic>\n                    2010)\n                  <\/jats:p>","DOI":"10.1111\/j.1349-7006.2010.01546.x","type":"journal-article","created":{"date-parts":[[2010,3,15]],"date-time":"2010-03-15T05:54:10Z","timestamp":1268632450000},"page":"1347-1353","source":"Crossref","is-referenced-by-count":16,"title":["<i>Bcl11b<\/i>\n                    heterozygosity promotes clonal expansion and differentiation arrest of thymocytes in \u03b3\u2010irradiated mice"],"prefix":"10.1111","volume":"101","author":[{"given":"Rieka","family":"Go","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Satoshi","family":"Hirose","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shinichi","family":"Morita","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takashi","family":"Yamamoto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshinori","family":"Katsuragi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yukio","family":"Mishima","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ryo","family":"Kominami","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2010,5,19]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)81683-9"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrc795"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2003-12-4111"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature05690"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature06866"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-06-3126"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-291X(02)03069-3"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1349-7006.2006.00218.x"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2007.02.003"},{"key":"e_1_2_6_11_2","first-page":"5329","article-title":"The 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